Pathogenicity of Different Baboon Herpesvirus Papio 2 Isolates Is Characterized by either Extreme Neurovirulence or Complete Apathogenicity
| Autor: | Jerry W. Ritchey, Katie A. Ealey, Richard Eberle, Darla H. Black, Kristin M. Rogers |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Simplexvirus
food.ingredient Immunology Virulence Enzyme-Linked Immunosorbent Assay Simian Antibodies Viral Virus Replication Microbiology Virus Mice food Virology biology.animal Chlorocebus aethiops Animals Vero Cells Cytopathic effect Mice Inbred BALB C biology biology.organism_classification Spinal Cord Viral replication Immunoglobulin G Insect Science Vero cell Pathogenesis and Immunity Female Papio Baboon |
| Zdroj: | Journal of Virology. 77:10731-10739 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.77.20.10731-10739.2003 |
| Popis: | In comparisons of the pathogenicity of simian alphaherpesviruses in mice, two isolates of the baboon virus HVP2 were nearly as lethal as monkey B virus, a biological safety level 4 agent (J. W. Ritchey, K. A. Ealey, M. Payton, and R. Eberle, J. Comp. Pathol. 127: 150-161, 2002). To confirm these results, mice were inoculated intramuscularly with 10 5 PFU of HVP2 isolates obtained from different baboon subspecies and primate centers. Some of the HVP2 isolates (6 of 13) caused paralysis and death in the mice, while 7 of 13 HVP2 isolates produced no clinical signs of disease. The apathogenic HVP2 isolates (HVP2 ap ) induced only low levels of serum antiviral immunoglobulin G relative to levels observed in sera from mice infected with the neurovirulent isolates of HVP2 (HVP2 nv ). Histological examination of tissues from mice inoculated with HVP2 nv isolates showed extensive neural tissue destruction, while mice infected with HVP2 ap isolates showed no lesions. Tissue samples collected at 48-h intervals postinfection suggested that HVP2 ap isolates failed to replicate at the site of inoculation. There was no significant difference in the in vitro replication, plaque size, or cytopathic effect morphology of HVP2 ap versus HVP2 nv isolates. While HVP2 isolates replicated better in Vero monkey kidney cells than in murine L cells, plaquing efficiency of individual isolates did not correlate with the dichotomous pathogenic properties seen in mice. Phylogenetic analyses of both coding and intergenic regions (US4-6) of the HVP2 genome separated isolates into two distinct clades that correlated with the two in vivo virulence phenotypes. Taken together, these results demonstrate that two subtypes of HVP2 exist that are very closely related but differ dramatically in their ability to cause disease in a murine model. |
| Databáze: | OpenAIRE |
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