Application of cider by-products for medium chain length polyhydroxyalkanoate production by Pseudomonas putida KT2440
| Autor: | James Winterburn, Arantxa Eceiza, Phavit Wongsirichot, Aloña Retegi, Nagore Gabilondo, Leire Urbina, Maria Angeles Corcuera |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Polymers and Plastics biology Chemistry Organic Chemistry Pomace General Physics and Astronomy Biomass Substrate (chemistry) Fructose engineering.material biology.organism_classification 01 natural sciences Environmentally friendly Pseudomonas putida Polyhydroxyalkanoates 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 010608 biotechnology Materials Chemistry engineering Biopolymer Food science |
| Zdroj: | Urbina, L, Wongsirichot, P, Corcuera, M Á, Gabilondo, N, Eceiza, A, Winterburn, J & Retegi, A 2018, ' APPLICATION OF CIDER BY-PRODUCTS FOR MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATE PRODUCTION BY PSEUDOMONAS PUTIDA KT2440 ', European Polymer Journal, vol. 108, pp. 1-9 . https://doi.org/10.1016/j.eurpolymj.2018.08.020 |
| ISSN: | 0014-3057 |
| Popis: | In this study the technical feasibility of using cider by-products as a substrate for the production of medium chain length polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas putida KT2440 is demonstrated. The untreated apple pomace was found to be composed of two main residual sugars, glucose and fructose, making it a promising cheap growth substrate. A PHA concentration of 1.1 g L−1 and a PHA accumulation of 25.5% were obtained in flask experiments using apple pomace as the sole carbon source without any pretreatment, employing a fed batch strategy. The biopolymer was extracted from the biomass and a detailed characterization was carried out. The solution-casted film was found to be a mcl-PHA copolymer composed by different saturated and unsaturated ester monomers with elastomeric characteristics making it suitable for applications in biomedicine or the processing of value added co-products such as environmentally friendly adhesives or biodegradable films for agricultural purposes. |
| Databáze: | OpenAIRE |
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