Evaluation of Macroalgae Sulfated Polysaccharides on the Leishmania (L.) amazonensis Promastigote
| Autor: | Henrique Hessel Gaeta, Daniel Bristot, Wladimir Ronald Lobo Farias, Selma Dzimidas Rodrigues, Marcos H. Toyama, Daniela de Oliveira Toyama, Camila L. Pires |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Univ Prebiteriana Mackenzie, Universidade Federal do Ceará (UFC) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
macroalgae
Leishmania (L.) amazonensis Cell Survival Antiprotozoal Agents Pharmaceutical Science Polysaccharide sulfated polysaccharides Article Microbiology Cell Line Solieria filiformis Polysaccharides Drug Discovery parasitic diseases Caulerpa racemosa Cytotoxic T cell Animals Botryocladia occidentalis Cytotoxicity Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 EC50 chemistry.chemical_classification Leishmania biology Sulfates Macrophages Cell Membrane In vitro toxicology biology.organism_classification Seaweed In vitro Gracilaria caudate chemistry Biochemistry lcsh:Biology (General) Cell culture Macrophages Peritoneal |
| Zdroj: | Marine Drugs Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Marine Drugs; Volume 11; Issue 3; Pages: 934-943 Marine Drugs, Vol 11, Iss 3, Pp 934-943 (2013) |
| ISSN: | 1660-3397 |
| Popis: | Made available in DSpace on 2014-12-03T13:11:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-03-01Bitstream added on 2014-12-03T13:23:33Z : No. of bitstreams: 1 WOS000316607800023.pdf: 364360 bytes, checksum: fdfdf38afecf4c0522eef5897bd5ac91 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The sulfated polysaccharides from Solieria filiformis (Sf), Botryocladia occidentalis (Bo), Caulerpa racemosa (Cr) and Gracilaria caudata (Gc) were extracted and extensively purified. These compounds were then subjected to in vitro assays to evaluate the inhibition of these polysaccharides on the growth of Leishmania (L.) amazonensis promastigotes. Under the same assay conditions, only three of the four sulfated polysaccharides were active against L. amazonensis, and the polysaccharide purified from Cr was the most potent (EC50 value: 34.5 mu g/mL). The polysaccharides derived from Bo and Sf demonstrated moderate anti-leishmanial activity (EC50 values of 63.7 mu g/mL and 137.4 mu g/mL). In addition, we also performed in vitro cytotoxic assays toward peritoneal macrophages and J774 macrophages. For the in vitro cytotoxicity assay employing J774 cells, all of the sulfated polysaccharides decreased cell survival, with CC50 values of 27.3 mu g/mL, 49.3 mu g/mL, 73.2 mu g/mL, and 99.8 mu g/mL for Bo, Cr, Gc, and Sf, respectively. However, none of the sulfated polysaccharides reduced the cell growth rate of the peritoneal macrophages. These results suggest that macroalgae contain compounds with various chemical properties that can control specific pathogens. According to our results, the assayed sulfated polysaccharides were able to modulate the growth rate and cell survival of Leishmania (L.) amazonensis promastigotes in in vitro assays, and these effects involved the interaction of the sulfated polysaccharides on the cell membrane of the parasites. State Univ Sao Paulo Julio Mesquita Filho, UNESP, Sao Vicente Unit, BR-11330900 Sao Vicente, SP, Brazil Univ Prebiteriana Mackenzie, Ctr Biol & Hlth Sci, BR-01302907 Sao Paulo, Brazil Univ Fed Ceara, Pici Unit, UFC, BR-60356000 Fortaleza, Ceara, Brazil State Univ Sao Paulo Julio Mesquita Filho, UNESP, Sao Vicente Unit, BR-11330900 Sao Vicente, SP, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|