Phenolic profile, safety assessment, and anti-inflammatory activity of Salvia verbenaca L
| Autor: | Nadjat Righi, Abderrahmane Baghiani, Amirouche Deghima, Susana M. Cardoso, Elisabete Coelho, Pedro A.R. Fernandes, Faiza Baali, Manuel A. Coimbra, Sabah Boumerfeg |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
medicine.drug_class medicine.medical_treatment Anti-Inflammatory Agents Salvia Xylenes Kidney Anti-inflammatory Antioxidants 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine food Phenols In vivo Drug Discovery medicine Animals Edema 030304 developmental biology Pharmacology 0303 health sciences biology Traditional medicine Chemistry Plant Extracts Rosmarinic acid Plant Components Aerial biology.organism_classification food.food Liver 030220 oncology & carcinogenesis Larva Toxicity Salvia verbenaca Artemia salina |
| Zdroj: | Journal of ethnopharmacology. 272 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Salvia species are known to have anti-inflammatory properties, and are traditionally used for healing purposes. Salvia verbenaca is an Algerian plant used for healing wounds and ulcers. Aim of the study This work aims to assess the acute and subacute safety of S. verbenaca and its possible anti-inflammatory activity as a mechanism contributing to its traditional applications. Materials and methods Lethal toxicity of S. verbenaca hydromethanolic extract was evaluated against Artemia salina larvae, while acute and subacute toxicity were orally tested on mice. The anti-inflammatory activity was screened ex vivo using membrane stabilization and in vivo using xylene induced ear edema as an acute inflammation model. The antiradical, reducing power and iron chelating activities of S. verbenaca were also investigated in vitro, and phenolic compounds were determined using UHPLC-DAD-ESI-MSn. Results Salvia verbenaca extract contained high amounts of phenolic compounds (206 μg GAE/mg extract). The in vitro antioxidant activity showed promising radical scavenging ability, iron chelating (IC50: 189 μg/mL), reducing power and strong anti-lipid-peroxidation effect (IC50: 111 μg/mL). The extract had potential cytotoxic effect against Artemia salina larvae (LC50: 30 μg/mL), but did not exhibit any acute/subacute toxicity effect on mice. Salvia verbenaca inhibited hypotonic and heat induced hemolysis and also reduced 50% of xylene induced ear edema at 600 mg/kg bw. Rosmarinic acid and caffeoylmalic acid were identified as the major compounds. Conclusion Salvia verbenaca hydromethanolic extract was found to be safe at acute and subacute levels. Its in vitro/in vivo antioxidant activity, membrane stabilizing properties and anti-inflammatory activity may be an important aspect of its wound healing and anti-ulcer traditional use. |
| Databáze: | OpenAIRE |
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