A Neonatal Mouse Spinal Cord Compression Injury Model
| Autor: | Mark Züchner, Jean-Luc Boulland, Joel C. Glover |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
General Chemical Engineering medicine.medical_treatment Poison control Hindlimb General Biochemistry Genetics and Molecular Biology neuroscience surgery Mice 03 medical and health sciences 0302 clinical medicine Spinal cord compression Neuroplasticity medicine Animals clip Spinal cord injury General Immunology and Microbiology business.industry General Neuroscience Laminectomy Spinal cord medicine.disease compression spinal cord injury Disease Models Animal Electrophysiology 030104 developmental biology medicine.anatomical_structure Animals Newborn plasticity Anesthesia neonatal mouse Medicine Issue 109 business Spinal Cord Compression Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| DOI: | 10.3791/53498 |
| Popis: | Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal cord. A major current area of research is focused on the mechanisms of adaptive plasticity that underlie spontaneous or induced functional recovery following SCI. Spontaneous functional recovery is reported to be greater early in life, raising interesting questions about how adaptive plasticity changes as the spinal cord develops. To facilitate investigation of this dynamic, we have developed a SCI model in the neonatal mouse. The model has relevance for pediatric SCI, which is too little studied. Because neural plasticity in the adult involves some of the same mechanisms as neural plasticity in early life(1), this model may potentially have some relevance also for adult SCI. Here we describe the entire procedure for generating a reproducible spinal cord compression (SCC) injury in the neonatal mouse as early as postnatal (P) day 1. SCC is achieved by performing a laminectomy at a given spinal level (here described at thoracic levels 9-11) and then using a modified Yasargil aneurysm mini-clip to rapidly compress and decompress the spinal cord. As previously described, the injured neonatal mice can be tested for behavioral deficits or sacrificed for ex vivo physiological analysis of synaptic connectivity using electrophysiological and high-throughput optical recording techniques(1). Earlier and ongoing studies using behavioral and physiological assessment have demonstrated a dramatic, acute impairment of hindlimb motility followed by a complete functional recovery within 2 weeks, and the first evidence of changes in functional circuitry at the level of identified descending synaptic connections(1). |
| Databáze: | OpenAIRE |
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