New WGS data and annotation of the heterosomal vs. autosomal localization of Ostrinia scapulalis (Lepidoptera, Crambidae) nuclear genomic scaffolds
| Autor: | Sabine Nidelet, Réjane Streiff, Louise Brousseau |
|---|---|
| Přispěvatelé: | Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ANR Adapt-Ome - French National Research Agency : ANR-13-BSV7-0012, Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Rok vydání: | 2018 |
| Předmět: |
Ostrinia scapulalis
Genome NGS HiSeq2500 Depth analysis AD-ratio Structural annotation Sex-chromosome Z-heterosome Autosomes 0301 basic medicine Computational biology lcsh:Computer applications to medicine. Medical informatics Deep sequencing Ostrinia 03 medical and health sciences Annotation Biologie animale lcsh:Science (General) Gene Animal biology Multidisciplinary Autosome biology Shotgun sequencing [SDV.BA]Life Sciences [q-bio]/Animal biology biology.organism_classification Philosophy 030104 developmental biology lcsh:R858-859.7 lepidoptera crambidae Heterogametic sex lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, 2018, 20, pp.644-648. ⟨10.1016/j.dib.2018.08.011⟩ Data in Brief, Elsevier, 2018, 20, pp.644-648. ⟨10.1016/j.dib.2018.08.011⟩ Data in Brief, Vol 20, Iss, Pp 644-648 (2018) Data in Brief (20), 644-648. (2018) |
| ISSN: | 2352-3409 |
| DOI: | 10.1016/j.dib.2018.08.011 |
| Popis: | Here, we introduce new whole-genome shotgun sequencing and annotation data describing the autosomal vs. Z-heterosomal localization of nuclear genomic scaffolds of the moth species Ostrinia scapulalis. Four WGS libraries (corresponding to 2 males and 2 females) were sequenced with an Illumina HiSeq2500 sequencing technology, and the so-called ‘AD-ratio’ method was applied to distinguish between autosomal and Z-heterosomal scaffolds based on sequencing depth comparisons between homogametic (male) and heterogametic (female) libraries. A total of 25,760 scaffolds (corresponding to 341.69 Mb) were labelled as autosomal and 1273 scaffolds (15.29 Mb) were labelled as Z-heterosomal, totaling about 357 Mb. Besides, 4874 scaffolds (29.07 Mb) remain ambiguous because of a lack of AD-ratio reproducibility between the two replicates. The annotation method was evaluated a posteriori, by comparing depth-based annotation with the exact localization of known genes. Raw genomic data have been deposited and made accessible via the EMBL ENA BioProject id PRJEB26557. Comprehensive annotation is made accessible via the LepidoDB database (http://bipaa.genouest.org/sp/ostrinia_scapulalis/download/genome/v1.2/). Keywords: Ostrinia scapulalis, Genome, NGS, HiSeq2500, Depth analysis, AD-ratio, Structural annotation, Sex-chromosome, Z-heterosome, Autosomes |
| Databáze: | OpenAIRE |
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