Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani

Autor: William H. Wheat, Mary Jackson, Emily K Luna, Courtney E. Jahn, John J. Long, Jan E. Leach
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Microorganism
Heterotroph
Biological pest control
lcsh:Medicine
Acanthamoeba
macromolecular substances
Biology
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
Dictyostelium discoideum
Rhizoctonia
Microbiology
Rhizoctonia solani
Crop
03 medical and health sciences
Sheath blight
Cell Wall
Antibiosis
Free-living amoebae
Dictyostelium
Pest Control
Biological
lcsh:Science (General)
Pathogen
lcsh:QH301-705.5
Mycelium
Plant Diseases
Hartmannella
fungi
lcsh:R
food and beverages
Oryza
General Medicine
biology.organism_classification
bacterial infections and mycoses
equipment and supplies
Coculture Techniques
Research Note
030104 developmental biology
Biological Control Agents
lcsh:Biology (General)
Vermamoeba
010606 plant biology & botany
lcsh:Q1-390
Zdroj: BMC Research Notes, Vol 12, Iss 1, Pp 1-7 (2019)
BMC Research Notes
ISSN: 1756-0500
DOI: 10.1186/s13104-019-4802-2
Popis: Objective Rhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are available to control this pathogen and classic single gene resistance mechanisms in rice plants have not been identified. We hypothesize that alternate means of control are available in the environment including free-living amoebae. Amoebae are soil-, water- and air-borne microorganisms that are predominantly heterotrophic. Many amoeba species are mycophagous, and several harm their prey using mechanisms other than phagocytosis. Here, we used light and scanning electron microscopy to survey the interactions of R. solani with four amoeba species, with the goal of identifying amoebae species with potential for biocontrol. Results We observed a wide range of responses during interactions of R. solani with four different free-living amoebae. Two Acanthamoeba species encyst in co-cultures with R. solani at higher rates than medium without R. solani. Vermamoeba vermiformis (formerly Hartmanella vermiformis) attach to R. solani mycelium and are associated with mycelial shriveling and perforations of fungal cell walls, indicating an antagonistic interaction. No phenotypic changes were observed in co-cultures of Dictyostelium discoideum and R. solani.
Databáze: OpenAIRE
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