Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility
| Autor: | Norma S. Pérez-Gallardo, Yolik Ramírez-Marín, David Eduardo Abad-Contreras, Francisco Drusso Martinez-Garcia, Martin C. Harmsen, Jorge Luna del Villar-Velasco, Lorena Villafuerte-García, Gonzalo Torres-Villalobos, David M Giraldo-Gomez, Jesús Tapia-Jurado, Leonardo A. Rodriguez-Lopez, M. Cristina Piña-Barba, Miguel Angel Mercado, Martha Ustarroz-Cano, Dulce Maria Puente-Guzmán |
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| Přispěvatelé: | Restoring Organ Function by Means of Regenerative Medicine (REGENERATE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
Pathology medicine.medical_specialty Scaffold H&E stain 02 engineering and technology Matrix (biology) Article Extracellular matrix 03 medical and health sciences Cytokeratin chemistry.chemical_compound bile duct medicine General Materials Science DAPI 030304 developmental biology matrix scaffold Microscopy QC120-168.85 0303 health sciences Decellularization Bile duct Chemistry QH201-278.5 Engineering (General). Civil engineering (General) 021001 nanoscience & nanotechnology extracellular matrix (ECM) TK1-9971 medicine.anatomical_structure Descriptive and experimental mechanics decellularization Electrical engineering. Electronics. Nuclear engineering TA1-2040 0210 nano-technology |
| Zdroj: | Materials Materials, Vol 14, Iss 3099, p 3099 (2021) Materials, 14(11):3099. IOP PUBLISHING LTD Volume 14 Issue 11 |
| ISSN: | 1996-1944 |
| Popis: | Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4’-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H& E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility. |
| Databáze: | OpenAIRE |
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