| Autor: |
Elena Gkantzou, Anastasia Skonta, Aliki Tsakni, Angeliki Polydera, Dimitrios Moschovas, Konstantinos Spyrou, Apostolos Avgeropoulos, Dimitrios Gournis, Dimitra Houhoula, Haralambos Stamatis |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of Biotechnology. 350:75-85 |
| ISSN: |
0168-1656 |
| DOI: |
10.1016/j.jbiotec.2022.04.005 |
| Popis: |
Process sustainability of biocatalytic processes is significantly empowered with the use of continuous-flow technologies that offer high productivity, minimal wastes and low volumetric consumption. Combining microreactor design with 3D printing technology can broaden the engineering potentials. This work proposes a protocol to modify the surface of 3D-printed PLA scaffolds, based on chitosan deposition. Mimicking the concept of microplates, multi-well plates were designed to facilitate parameter testing. Immobilization of laccase from Trametes versicolor was successfully performed, while chitosan and cross-linker concentration and incubation time were optimized. Τhe developed protocol was applied for the continuous flow bioconversion of hydroxyyrosol, yielding a TTN of 438.6 × 10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect