Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton
Autor: | Hyun Bi Kim, Hyeong Kyu Lee, Dae-Soo Kim, Seung Hyun Son, Hyeran Kim, Jeongyeo Lee, Sung Ran Min, Haeng Soon Lee, Hyun Soon Kim, Jaeeun Jung, Kun Hyang Park, Sang Un Park, Young-Hee Noh, Cha Young Kim |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Article Subject Phytochemicals lcsh:Medicine Genes Plant 01 natural sciences lcsh:Technology General Biochemistry Genetics and Molecular Biology p-Coumaric acid Ferulic acid 03 medical and health sciences chemistry.chemical_compound Flavonols Chlorogenic acid Phenols Gene Expression Regulation Plant Caffeic acid lcsh:Science Chromatography High Pressure Liquid General Environmental Science chemistry.chemical_classification Sophora flavescens biology Chemistry lcsh:T Plant Extracts Gene Expression Profiling lcsh:R General Medicine Phenolic acid biology.organism_classification Biosynthetic Pathways 030104 developmental biology Biochemistry Organ Specificity lcsh:Q Kaempferol Transcriptome Sophora 010606 plant biology & botany Research Article |
Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 2018 (2018) |
ISSN: | 1537-744X 2356-6140 |
Popis: | Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally. |
Databáze: | OpenAIRE |
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