Silk fibroin nanoparticles for celecoxib and curcumin delivery: ROS-scavenging and anti-inflammatory activities in an in vitro model of osteoarthritis
| Autor: | Giuseppe Tripodo, Elia Bari, Barbara Crivelli, Sara Perteghella, Michela Mocchi, Adriele Prina-Mello, Maria Luisa Torre, Laura Catenacci, Milena Sorrenti, Silvio Faragò |
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| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
Curcumin medicine.drug_class Cell Survival medicine.medical_treatment Anti-Inflammatory Agents Pharmaceutical Science Fibroin 02 engineering and technology Pharmacology 030226 pharmacology & pharmacy Hemolysis Anti-inflammatory Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Osteoarthritis medicine Distribution (pharmacology) Humans Viability assay Particle Size Cytotoxicity Cells Cultured Drug Synergism General Medicine 021001 nanoscience & nanotechnology Drug Liberation chemistry Celecoxib Nanoparticles 0210 nano-technology Fibroins Reactive Oxygen Species Biotechnology medicine.drug |
| Zdroj: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 137 |
| ISSN: | 1873-3441 |
| Popis: | This paper aims at demonstrating silk fibroin nanoparticles (SFNs) promote anti-inflammatory properties of celecoxib (CXB) or curcumin (CUR), and could be exploited for osteoarthritis (OA) treatment. Nanoparticles were prepared by desolvation method and physico-chemically characterized (FT-IR, DSC, TGA, SEM, size distribution and drug release); empty and drug loaded nanoparticles were tested for their ROS-scavenging activity, hemolytic properties, cytotoxicity, and anti-inflammatory potency in an OA in vitro model. Results indicate that a controlled drug release has been achieved by varying the drug loading. Curcumin plus SFNs exhibited a synergistic antioxidant effect, while CXB was, in some manner, inhibitory. Both free drugs resulted highly cytotoxic while cell viability reached high values when encapsulated in SFNs. No appreciable differences in anti-inflammatory activity was evidenced between CUR loaded SFNs and CXB. In conclusion, SFNs is an optimal carrier to improve cyto- and hemo-compatibility of both CUR and CXB. |
| Databáze: | OpenAIRE |
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