Transforming Growth Factor β/activin signalling induces epithelial cell flattening during Drosophila oogenesis
| Autor: | Jean-Luc Duteyrat, Isabelle Brigaud, Jean-Louis Couderc, Sandrine Le Bail, Muriel Grammont, Julien Chlasta |
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| Přispěvatelé: | École normale supérieure - Lyon (ENS Lyon), Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, École normale supérieure de Lyon (ENS de Lyon), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
QH301-705.5
[SDV]Life Sciences [q-bio] Science Cell Biology Bioinformatics General Biochemistry Genetics and Molecular Biology Epithelium Cell biology Adherens junction TGFβ medicine.anatomical_structure Myosin medicine Epithelial cell flattening Biology (General) Signal transduction General Agricultural and Biological Sciences Cytoskeleton Actin Research Article Transforming growth factor |
| Zdroj: | Biology Open, Vol 4, Iss 3, Pp 345-354 (2015) Biology Open Biology Open, Royal Society, 2015, 4 (3), pp.345-54. ⟨10.1242/bio.201410785⟩ Biology Open, 2015, 4 (3), pp.345-54. ⟨10.1242/bio.201410785⟩ |
| ISSN: | 2046-6390 |
| Popis: | Although the regulation of epithelial morphogenesis is essential for the formation of tissues and organs in multicellular organisms, little is known about how signalling pathways control cell shape changes in space and time. In the Drosophila ovarian epithelium, the transition from a cuboidal to a squamous shape is accompanied by a wave of cell flattening and by the ordered remodelling of E-cadherin-based adherens junctions. We show that activation of the TGFβ pathway is crucial to determine the timing, the degree and the dynamic of cell flattening. Within these cells, TGFβ signalling controls cell-autonomously the formation of Actin filament and the localisation of activated Myosin II, indicating that internal forces are generated and used to remodel AJ and to promote cytoskeleton rearrangement. Our results also reveal that TGFβ signalling controls Notch activity and that its functions are partly executed through Notch. Thus, we demonstrate that the cells that undergo the cuboidal-to-squamous transition produce active cell-shaping mechanisms, rather than passively flattening in response to a global force generated by the growth of the underlying cells. Thus, our work on TGFβ signalling provides new insights into the mechanisms through which signal transduction cascades orchestrate cell shape changes to generate proper organ structure. |
| Databáze: | OpenAIRE |
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