Resistance to treatment in Kala-azar: speciation of isolates from northeast India
| Autor: | Richard T. Kenney, Ramesh Kumar, Kiran Pathak-Tripathi, Kalpana Pai, Shyam Sundar, Mitali Ray, Albert A. Gam |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Leishmania tropica Adolescent Antiprotozoal Agents Drug Resistance Leishmania donovani Antibodies Protozoan India Drug resistance Parasitemia Microbiology Virology parasitic diseases medicine Animals Humans Typing Child biology DNA Kinetoplast Kinetoplastida Leishmaniasis Electrophoresis Cellulose Acetate DNA Protozoan Middle Aged medicine.disease biology.organism_classification Infectious Diseases Visceral leishmaniasis Antimony Sodium Gluconate Kinetoplast Immunology Leishmaniasis Visceral Female Parasitology |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 65:193-196 |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Kala-azar in India is becoming increasingly difficult to treat, which may be due to the presence of species other than Leishmania donovani; Leishmania tropica was reported to cause the same clinical syndrome in the area. Over the past 3 years, we have collected samples from 241 patients with visceral leishmaniasis from across the region. Of the 189 isolates that grew on diphasic medium, 159 were successfully transferred to liquid medium for typing. Clinically, 80% of these were resistant to antimony. Lipophosphoglycan-specific monoclonal antibodies were used to distinguish the 2 species by agglutination of promastigotes; all 159 were shown to be L. donovani. Eighty-three isolates were confirmed to be L. donovani by isoenzyme analysis, by amplification of kinetoplast DNA, or both, in comparison with multiple reference strains; none were L. tropica. Thus, the rising incidence of clinical resistance to treatment is unlikely to be due to a different species causing kala-azar in north Bihar. |
| Databáze: | OpenAIRE |
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