Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation
| Autor: | I. K. Oyewusi, E. O. Adekunle, J. A. Oyewusi, O. A. Isah, A. O. Ladokun, Matthew A. Adeleke, O.E. Oke, John Adesanya Abiona, Olajide Mark Sogunle, O. M. Onagbesan, M. O. Abioja, J. O. Daramola |
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| Rok vydání: | 2016 |
| Předmět: |
General Veterinary
Extender 0402 animal and dairy science Semen 04 agricultural and veterinary sciences Orange (colour) Biology biology.organism_classification 040201 dairy & animal science Sperm Cryopreservation law.invention 03 medical and health sciences 0302 clinical medicine law 030220 oncology & carcinogenesis Botany Animal Science and Zoology Food science Acrosome Ananas Citrus × sinensis |
| Zdroj: | Animal Reproduction. 13:7-13 |
| ISSN: | 1984-3143 |
| DOI: | 10.21451/1984-3143-ar726 |
| Popis: | The present study was conducted to determine the effects of adding fruit-juices to semen extenders on viability of buck spermatozoa during cryopreservation in two studies. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-egg yolk based extenders. In study I, the diluted semen samples were supplemented with orange ( Citrus sinensis ), cucumber ( Cucumis sativus ) and pineapple ( Ananas comosus ) juices (2.5, 5, 7.5 and 10 ml/100 ml). In study II, the diluted semen samples were supplemented with 5 ml/100 ml of different combinations of fruit-juices consisting of pineapple and orange juices (TEYPO) at a 1:1 ratio, pineapple and cucumber juices (TEYPC) at a 1:1 ratio, orange and cucumber juices (TEYOC) at a 1:1 ratio, and pineapple, orange and cucumber juices (TEYPOC) at a 1:1:1 ratio, respectively. The diluted semen samples were cryopreserved and thereafter evaluated for sperm viability parameters. The extenders supplemented with 7.5 and 10% orange, and 10% pineapple had higher (P < 0.05) motility compared to the control. There was higher (P < 0.05) acrosome integrity in extenders supplemented with orange and pineapple at all levels compared to the control while cucumber at 10% had higher (P < 0.05) acrosome integrity compared to other levels and the control. The extenders supplemented with cucumber, orange and pineapple had higher (P < 0.05) membrane integrity compared to the control except at 7.5% of extender supplemented with cucumber. Similarly, the extenders supplemented with cucumber, orange and pineapple had lower (P < 0.05) sperm abnormality compared to the control. The extenders supplemented with cucumber, orange and pineapple at 2.5, 10 and 7.5% respectively had lower (P < 0.05) concentrations of MDA compared to other levels and the control. There was higher motility and acrosome integrity in extenders supplemented with different combinations of fruit-juices (P < 0.05) compared to the control. TEYPC had higher (P < 0.05) membrane integrity and lower (P < 0.05) concentrations of MDA compared to the control. The findings revealed that extenders supplemented with orange and pineapple at 10% consistently improved motility, acrosome integrity and membrane integrity, and reduced sperm abnormality compared to the control while no particular combination of these fruit-juices was consistently superior in all the parameters. |
| Databáze: | OpenAIRE |
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