HPLC Method for Chemical Fingerprinting of Guggul (Commiphora wightii)--Quantification of E- and Z-Guggulsterones and Detection of Possible Adulterants
| Autor: | Rida Ahmed, Yan-Hong Wang, Ikhlas A. Khan, Troy J. Smillie, Zulfiqar Ali |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
Adulterant Chromatography biology Calibration curve Organic Chemistry Pharmaceutical Science biology.organism_classification High-performance liquid chromatography Analytical Chemistry Commiphora wightii chemistry.chemical_compound Acetic acid Complementary and alternative medicine chemistry Pregnenediones Drug Discovery Molecular Medicine Guggulsterone Hplc method Chemical fingerprinting Commiphora Chromatography High Pressure Liquid Resins Plant |
| Zdroj: | Planta medica. 82(4) |
| ISSN: | 1439-0221 |
| Popis: | A reversed-phase high-performance liquid chromatography method was developed for the chemical fingerprinting of Commiphora wightii gum resin (guggul). This method was also used for the quantification of E- and Z-guggulsterones in different C. wightii gum resin samples. The analysis was conducted on a high-performance liquid chromatography system with a reversed-phase column using a gradient elution comprised of water and acetonitrile with 0.1 % acetic acid, and a run time of 50 min at 38 °C. The calibration curve of E- and Z-guggulsterones showed good linearity with a standard correlation coefficient > 0.999, which is within the established range (0.5–250 µg/mL). Twelve marker compounds were selected and successfully analyzed by this method in 22 C. wightii gum resin samples along with 9 gum resin samples of possible adulterant species. |
| Databáze: | OpenAIRE |
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