Transgenic labeling of hair cells in the zebrafish acousticolateralis system
| Autor: | Jessica M. Baucom, Shraddha D. Jani, Yaneth Castellanos, A. J. Hudspeth, Yukako Asai, Gustavo Gomez, James M. McClintock, Brian M. McDermott, Catherine J. Starr |
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| Rok vydání: | 2010 |
| Předmět: |
Embryo
Nonmammalian Transgene Green Fluorescent Proteins Article Green fluorescent protein Animals Genetically Modified Hair Cells Auditory Gene expression Genetics medicine Animals Inner ear Transgenes Molecular Biology Zebrafish Gene biology Gene Expression Regulation Developmental Zebrafish Proteins biology.organism_classification Molecular biology Transgenesis Parvalbumins medicine.anatomical_structure biology.protein Parvalbumin Developmental Biology |
| Zdroj: | Gene Expression Patterns. 10:113-118 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.gep.2010.01.001 |
| Popis: | The zebrafish provides a useful experimental system for investigations of aural development. To permit the controlled expression of transgenes in developing hair cells, we isolated the genomic control regions of the parvalbumin 3a (pvalb3a) and parvalbumin 3b (pvalb3b) genes. Deletion analysis and somatic-cell transgenesis restricted the cis-acting control regions for hair cells to as little as 484 base pairs for pvalb3a and 650 base pairs for pvalb3b. Using both meganuclease-mediated and standard methods, we produced transgenic animals that transmit transgenes through their germ lines. These fish express GFP in hair cells in the inner ear and lateral line. Two stable transgenic lines express GFP prior to hair-bundle formation, so the associated promoter constructs are suitable for manipulating gene expression during bundle development. We additionally identified a transgenic line that offers variable labeling of supporting cells. |
| Databáze: | OpenAIRE |
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