Correlations of Promoter Methylation in WIF-1, RASSF1A, and CDH13 Genes with the Risk and Prognosis of Esophageal Cancer
| Autor: | Yong-Hui Li, Qiang Guo, Zhen-Qing Sun, Ting-Ting Li, Wen-Xue Zhang, He-Fei Li, Hai-Bo Wang, Sha-Sha Xiang |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine endocrine system Esophageal Neoplasms Repressor Biology Methylation 03 medical and health sciences 0302 clinical medicine Lab/In Vitro Research Risk Factors medicine Humans Genetic Predisposition to Disease Promoter Regions Genetic Gene Adaptor Proteins Signal Transducing Aged Cadherin Tumor Suppressor Proteins Wnt signaling pathway Promoter General Medicine DNA Methylation Middle Aged Esophageal cancer Cadherins Prognosis medicine.disease Molecular biology Repressor Proteins 030104 developmental biology 030220 oncology & carcinogenesis DNA methylation Cancer research Female |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.896877 |
| Popis: | BACKGROUND This study was designed to explore the correlations of promoter methylation in Wnt inhibitory factor-1 (WIF-1), ras-association domain family member 1A (RASSF1A), and Cadherin 13 (CDH13) genes with the risk and prognosis of esophageal cancer (EC). MATERIAL AND METHODS A total of 71 EC tissues from resection and 35 adjacent normal tissues were collected. Methylation status in the promoter region was detected by methylation- and non-methylation-specific primers. Corresponding mRNA levels were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Correlations between the methylations of these 3 genes and clinicopathologic characteristics were analyzed. Kaplan-Meier method and Cox regression model were used to investigate the relationships between WIF-1, RASSF1A, and CDH13 promoter methylations and the prognosis of EC. RESULTS Compared with adjacent normal tissues, the methylation frequencies of WIF-1, RASSF1A, and CDH13 genes were significantly higher but the mRNA levels of these 3 genes were significantly lower in EC tissues (all P |
| Databáze: | OpenAIRE |
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