Arabidopsis PROTEINS-ACYL TRANSFERASE4 mediates root hair growth
| Autor: | Fu-Rong Ge, Sha Li, Sen Chai, Zhi-Yuan Wan, Qiang-Nan Feng, Yan Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Arabidopsis Mutagenesis (molecular biology technique) Plant Science Root hair Root hair elongation Plant Roots 01 natural sciences 03 medical and health sciences GTP-Binding Proteins Genetics Arabidopsis thaliana Tip growth biology Arabidopsis Proteins Wild type Cell Biology Plants Genetically Modified biology.organism_classification Actins Cell biology 030104 developmental biology sense organs Acyltransferases Signal Transduction 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 90:249-260 |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/tpj.13484 |
| Popis: | Summary Polar growth of root hairs is critical for plant survival and necessitates fine-tuned ROP signaling. Multiple ROP regulators participate in root hair growth. However, protein S-acyl transferases (PATs), mediating the S-acylation and membrane partitioning of ROPs, are yet to be found. By using a reverse genetic approach, combining fluorescence probes, pharmacological drugs, site-directed mutagenesis, and genetic analysis with related root-hair mutants, we have identified and characterized an Arabidopsis PAT, which may be responsible for ROP2 S-acylation in root hairs. Specifically, functional loss of PAT4 resulted in reduced root hair elongation, which was rescued by a wild-type but not an enzyme-inactive PAT4. Membrane-associated ROP2 was significantly reduced in pat4, similar to that of S-acylation-deficient ROP2 in wild type. We further showed that PAT4 and SCN1, a ROP regulator, additively mediate ROP2 stability and targeting. Results presented indicate that PAT4-mediated S-acylation mediates the membrane association of ROP2 at the root hair apex and provide novel insights into the dynamic ROP signaling during plant tip growth. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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