Insulators to improve expression of a 3(')IgH LCR-driven reporter gene in transgenic mouse models
| Autor: | Michel Cogné, Yves Denizot, Laurence Guglielmi, Marc Le Bert, Véronique Truffinet |
|---|---|
| Přispěvatelé: | Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations (PMRIL), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2003 |
| Předmět: |
Genes
RAG-1 Gene Expression Biochemistry MESH: Mice Knockout Green fluorescent protein Mice 0302 clinical medicine Genes Reporter Transgenic mice MESH: Animals Transgenes Mice Knockout 0303 health sciences B-Lymphocytes MESH: Enhancer Elements (Genetics) Stem Cells MESH: Genes RAG-1 Globins medicine.anatomical_structure Enhancer Elements Genetic [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Luminescent Proteins Insulator Elements Immunoglobulin Heavy Chains MESH: Immunoglobulin Heavy Chains NLM MESH: Locus Control Region MESH: Gene Expression MESH: Mice Transgenic Transgene Green Fluorescent Proteins Biophysics MESH: Transgenes Mice Transgenic MESH: Stem Cells Biology GFP 03 medical and health sciences MESH: Green Fluorescent Proteins MESH: B-Lymphocytes medicine MESH: Globins Gene silencing Animals RNA Messenger Enhancer Molecular Biology Gene MESH: Mice B cell Locus control region 030304 developmental biology MESH: RNA Messenger Reporter gene MESH: Genes Reporter Cell Biology MESH: Insulator Elements Insulators Locus Control Region Molecular biology Luminescent Proteins 3′LCR 030215 immunology B lymphocytes |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2003, 307 (3), pp.466-71. ⟨10.1016/S0006-291X(03)01185-9⟩ |
| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1016/S0006-291X(03)01185-9⟩ |
| Popis: | A locus control region (LCR) containing four transcriptional enhancers lies downstream of the IgH chain locus. We studied transgenes carrying a 3(')IgH LCR-driven GFP reporter gene for expression and B cell differentiation stage specificity. We also compared transgenes that were or were not flanked by two copies of the beta-globin HS4 insulator, an element defined by its ability to protect transgenes from the influences of surrounding genes at the insertion site. Results indicate that insulators are instrumental in sustaining GFP expression in GFP-3(')LCR transgenic mice when they were included. Flow cytometry experiments reported a strictly B cell specific GFP expression from pre-B cells in bone marrow to mature B cells in spleen. Despite addition of 5(')HS4 insulators to the GFP-3(')LCR construct, complete transgene silencing occurred in some transgenic lines and was systematically observed in ageing animals from all lines. |
| Databáze: | OpenAIRE |
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