Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?

Autor: Bianca H. Ventura Fernandes, Natália Martins Feitosa, Ana Paula Barbosa, Camila Gasque Bomfim, Anali M.B. Garnique, Ivana F. Rosa, Maira S. Rodrigues, Lucas B. Doretto, Daniel F. Costa, Bruno Camargo-dos-Santos, Gabrielli A. Franco, João Favero Neto, Juliana Sartori Lunardi, Marina Sanson Bellot, Nina Pacheco Capelini Alves, Camila C. Costa, Mayumi F. Aracati, Letícia F. Rodrigues, Rafaela Hemily Cirilo, Raul Marcelino Colagrande, Francisco I.F. Gomes, Rafael T. Nakajima, Marco A.A. Belo, Percília Cardoso Giaquinto, Susana Luporini de Oliveira, Silas Fernandes Eto, Dayanne Carla Fernandes, Wilson G. Manrique, Gabriel Conde, Roberta R.C. Rosales, Iris Todeschini, Ilo Rivero, Edgar Llontop, Germán G. Sgro, Gabriel Umaji Oka, Natalia Fernanda Bueno, Fausto K. Ferraris, Mariana T.Q. de Magalhães, Renata J. Medeiros, Juliana M. Mendonça-Gomes, Mara Souza Junqueira, Kátia Conceição, Leticia Gomes de Pontes, Antonio Condino-Neto, Andrea C. Perez, Leonardo J.G. Barcellos, José Dias Correa Júnior, Erick Gustavo Dorlass, Niels O.S. Camara, Edison Luiz Durigon, Fernando Q. Cunha, Rafael H. Nóbrega, Glaucia M. Machado-Santelli, Chuck S. Farah, Flavio P. Veras, Jorge Galindo-Villegas, Letícia V. Costa-Lotufo, Thiago M. Cunha, Roger Chammas, Luciani R. Carvalho, Cristiane R. Guzzo, Guilherme Malafaia, Ives Charlie-Silva
Přispěvatelé: Universidade de São Paulo (USP), Universidade Federal do Rio de Janeiro (UFRJ), Universidade Estadual Paulista (UNESP), Brasil University, Federal University of Roraima, Butantan Institute, Federal University of Rondônia, Pontifícia Universidade Católica de Minas Gerais, Oswaldo Cruz Foundation, Universidade Federal de Minas Gerais (UFMG), National Institute for Quality Control in Health, University São Paulo, Federal University of Santa Maria, University of Passo Fundo, LIQBRA, Nord University, Disciplina de Endocrinologia do Departamento de Clinica Medica e Laboratório de Hormônios e Genética Molecular, Goiano Federal Institute
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: The Science of the Total Environment
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1879-1026
0048-9697
Popis: Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.
Graphical abstract Unlabelled Image
Databáze: OpenAIRE
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