Multinucleation-induced improvement of the spreading of transformed cells on the substratum
| Autor: | A. A. Stavrovskaya, Anna S. Serpinskaya, J M Vasiliev, L A Lyass, Vladimir I. Gelfand, Gel'fand Im, Alexander D. Bershadsky |
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| Rok vydání: | 1984 |
| Předmět: |
Cycloheximide
Biology Quail Avian sarcoma virus Cell Line Mice chemistry.chemical_compound L Cells Multinucleate Cricetinae Animals Intermediate filament Mitosis Cells Cultured Cell Nucleus Multidisciplinary Colcemid Demecolcine Culture Media Cell biology Cell Transformation Neoplastic Avian Sarcoma Viruses chemistry Cytoplasm Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 81:3098-3102 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.81.10.3098 |
| Popis: | Multinucleation of various cultured cells was produced by polyethylene glycol-induced fusion or by cytochalasin-induced block of mitosis. It was found that multinucleation induced by both methods considerably improved deficient spreading of all the tested transformed fibroblastic lines; average substratum area occupied by one cell and divided per number of nuclei was 2.0-2.5 times larger for multinucleated cells than for mononucleated ones. Improved spreading was accompanied by increased area of lamellar cytoplasm, increased number of focal contacts, and, in certain lines, by the appearance of actin bundles; numerous microtubules and intermediate filaments radiated from perinuclear zones into the lamellas of multinucleated cells. The number of cell-associated fibronectin fibrils was not increased by multinucleation. Cycloheximide did not prevent the improvement of spreading, suggesting that this effect was not due to any alterations of protein synthesis. Colcemid considerably decreased the effect of multinucleation but did not abolish it completely. It is suggested that increase of spreading is due to multinucleation-associated alterations of quantitative interrelationships between various cell components. One of these alterations is probably increased density of microtubules per unit length of outer cell edge. |
| Databáze: | OpenAIRE |
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