Studies on two groups of aminoacyl-tRNA synthetases from rat liver
| Autor: | G. Rezelman, J. Geels, Willy S. Bont |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
chemistry.chemical_classification
Aminoacyl tRNA synthetase Size-exclusion chromatography General Medicine Gradient centrifugation Biology Cell Fractionation Rats Amino acid Amino Acyl-tRNA Synthetases chemistry.chemical_compound Cytosol Liver chemistry Biochemistry Cytoplasm Rat liver Genetics Animals Acid treatment Molecular Biology Subcellular Fractions |
| Zdroj: | Molecular Biology Reports. 2:379-384 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/bf00366259 |
| Popis: | Aminoacyl-tRNA synthetases from rat-liver cytoplasm were fractionated into two groups, characterized by their sedimentation coefficients of about 20S and 5S, respectively. These two groups of synthetases could be isolated from postmicrosomal supernatant either by gradient centrifugation, by gel filtration or by acid treatment at pH 5.2. Both groups were required for maximal amino acid incorporation in a cell-free system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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