Production and enhancement of the acetylcholinesterase inhibitor, huperzine A, from an endophytic Alternaria brassicae AGF041

Autor:	Ashraf S. Ahmed, Amira G Zaki, Ola E A Al-Hagar, Einas H. El-Shatoury
Rok vydání:	2019
Předmět:	medicine.drug_class Applied Microbiology and Biotechnology Actinobacteria 03 medical and health sciences chemistry.chemical_compound Alkaloids medicine Prospective Studies Chromatography High Pressure Liquid Mycelium Huperzine A 030304 developmental biology 0303 health sciences Traditional medicine biology 030306 microbiology Alternaria Huperzia General Medicine Huperzia serrata biology.organism_classification Acetylcholinesterase Alternaria brassicae chemistry Acetylcholinesterase inhibitor Fermentation Cholinesterase Inhibitors Chromatography Thin Layer Sesquiterpenes Biotechnology medicine.drug
Zdroj:	Applied Microbiology and Biotechnology. 103:5867-5878
ISSN:	1432-0614 0175-7598
Popis:	Huperzine A (HupA) is a potent acetylcholinesterase (AChE) inhibitor of a great consideration as a prospective drug candidate for Alzheimer's disease treatment. Production of HupA by endophytes offers an alternative challenge to reduce the massive plant harvest needed to meet the increasing demand of HupA. In the current study, some endophytic fungal and actinobacterial isolates from the Chinese herb, Huperzia serrata, underwent liquid fermentation, alkaloid extraction, and screening for AChE inhibition and HupA production. Among these isolates, Alternaria brassicae AGF041 strain was the only positive strain for HupA production with the maximum AChE inhibition of 75.5%. Chromatographic analyses verified the identity of the produced HupA. The HupA production was efficiently maximized up to 42.89 μg/g of dry mycelia, after optimization of thirteen process parameters using multifactorial statistical approaches, Plackett-Burman and central composite designs. The statistical optimization resulted in a 40.8% increase in HupA production. This is the first report to isolate endophytic actinobacteria with anti-AChE activity from H. serrata, and to identify an endophytic fungus A. brassicae as a new promising start strain for a higher HupA yield.
Databáze:	OpenAIRE
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