Rcf1 and Rcf2, Members of the Hypoxia-Induced Gene 1 Protein Family, Are Critical Components of the Mitochondrial Cytochrome bc1-Cytochrome c Oxidase Supercomplex
| Autor: | Rosemary A. Stuart, Andrew I. Furness, Micaela Robb-McGrath, Vera Strogolova, Joshua Garlich |
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| Rok vydání: | 2012 |
| Předmět: |
Oxidase test
Saccharomyces cerevisiae Proteins Protein family Cytochrome Protein subunit Electron Transport Complex IV Articles Saccharomyces cerevisiae Cell Biology Biology Mitochondrion Models Biological Mitochondria Enzyme Activation Electron Transport Complex III Biochemistry Respirasome biology.protein Cytochrome c oxidase Hypoxia-Inducible Factor 1 Molecular Biology Protein Binding |
| Zdroj: | Molecular and Cellular Biology. 32:1363-1373 |
| ISSN: | 1098-5549 |
| Popis: | We report that Rcf1 (formerly Aim31), a member of the conserved hypoxia-induced gene 1 (Hig1) protein family, represents a novel component of the yeast cytochrome bc(1)-cytochrome c oxidase (COX) supercomplex. Rcf1 (respiratory supercomplex factor 1) partitions with the COX complex, and evidence that it may act as a bridge to the cytochrome bc(1) complex is presented. Rcf1 interacts with the Cox3 subunit and can do so prior to their assembly into the COX complex. A close proximity of Rcf1 and members of the ADP/ATP carrier (AAC) family was also established. Rcf1 displays overlapping function with another Hig1-related protein, Rcf2 (formerly Aim38), and their joint presence is required for optimal COX enzyme activity and the correct assembly of the cytochrome bc(1)-COX supercomplex. Rcf1 and Rcf2 can independently associate with the cytochrome bc(1)-COX supercomplex, indicating that at least two forms of this supercomplex exist within mitochondria. We provide evidence that the association with the cytochrome bc(1)-COX supercomplex and regulation of the COX complex are a conserved feature of Hig1 family members. Based on our findings, we propose a model where the Hig1 proteins regulate the COX enzyme activity through Cox3 and associated Cox12 protein, in a manner that may be influenced by the neighboring AAC proteins. |
| Databáze: | OpenAIRE |
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