Metformin-stimulated AMPK-α1 promotes microvascular repair in acute lung injury
| Autor: | Jaroslaw W. Zmijewski, Paul E. Wolkowicz, Judy Creighton, Ming-Yuan Jian, Mikhail Alexeyev |
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| Rok vydání: | 2013 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lipopolysaccharides Male Cell Membrane Permeability Endothelium Physiology Vascular permeability Lung injury Biology Pharmacology AMP-Activated Protein Kinases In Vitro Techniques Rats Sprague-Dawley AMP-activated protein kinase Cell Movement Physiology (medical) medicine Electric Impedance Animals RNA Small Interfering Lung Cells Cultured Respiratory Distress Syndrome Wound Healing AMPK Endothelial Cells Cell Biology Articles Ribonucleotides Aminoimidazole Carboxamide Metformin Rats Endothelial stem cell Enzyme Activation medicine.anatomical_structure Pyrimidines Gene Knockdown Techniques Immunology Microvessels biology.protein Pyrazoles Endothelium Vascular Ex vivo medicine.drug |
| Zdroj: | American journal of physiology. Lung cellular and molecular physiology. 305(11) |
| ISSN: | 1522-1504 |
| Popis: | Acute lung injury secondary to sepsis is a leading cause of mortality in sepsis-related death. Present therapies are not effective in reversing endothelial cell dysfunction, which plays a key role in increased vascular permeability and compromised lung function. AMP-activated protein kinase (AMPK) is a molecular sensor important for detection and mediation of cellular adaptations to vascular disruptive stimuli. In this study, we sought to determine the role of AMPK in resolving increased endothelial permeability in the sepsis-injured lung. AMPK function was determined in vivo using a rat model of endotoxin-induced lung injury, ex vivo using the isolated lung, and in vitro using cultured rat pulmonary microvascular endothelial cells (PMVECs). AMPK stimulation using N1-(α-d-ribofuranosyl)-5-aminoimidizole-4-carboxamide or metformin decreased the LPS-induced increase in permeability, as determined by filtration coefficient ( Kf) measurements, and resolved edema as indicated by decreased wet-to-dry ratios. The role of AMPK in the endothelial response to LPS was determined by shRNA designed to decrease expression of the AMPK-α1 isoform in capillary endothelial cells. Permeability, wounding, and barrier resistance assays using PMVECs identified AMPK-α1 as the molecule responsible for the beneficial effects of AMPK in the lung. Our findings provide novel evidence for AMPK-α1 as a vascular repair mechanism important in the pulmonary response to sepsis and identify a role for metformin treatment in the management of capillary injury. |
| Databáze: | OpenAIRE |
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