A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations
| Autor: | Margherita Anna Barracco, Rafael Diego Rosa, Javier Vernal, Paula Terra Bandeira, Gabriel Machado Matos, Natanael Dantas Farias, Aguinaldo R. Pinto, Hernán Terenzi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Hemocytes Antimicrobial peptides Aquatic Science Biology Immunofluorescence Gram-Positive Bacteria Microbiology Arthropod Proteins 03 medical and health sciences Penaeidae Gram-Negative Bacteria medicine Environmental Chemistry Animals Antiserum medicine.diagnostic_test 04 agricultural and veterinary sciences General Medicine biology.organism_classification Antimicrobial Crustacean Shrimp Anti-Bacterial Agents 030104 developmental biology Specific granule 040102 fisheries 0401 agriculture forestry and fisheries Bacteria Antimicrobial Cationic Peptides |
| Zdroj: | Fishshellfish immunology. 97 |
| ISSN: | 1095-9947 |
| Popis: | Crustins are cysteine-rich antimicrobial peptides (AMPs) widely distributed across crustaceans. From the four described crustin Types (I to IV), crustins from the subtype IIa are the most abundant and diverse members found in penaeid shrimp. Despite the critical role of Type IIa crustins in shrimp antimicrobial defenses, there is still limited information about their synthesis and antimicrobial properties. Here, we report the subcellular localization and the antibacterial spectrum of crusFpau, a Type IIa crustin from the pink shrimp Farfantepenaeus paulensis. The recombinantly expressed crusFpau showed antimicrobial activity against both Gram-positive and Gram-negative bacteria at low concentrations. Results from immunofluorescence using anti-rcrusFpau antiserum revealed that crusFpau is synthetized and stored by both granular and semigranular hemocytes, but not by hyaline cells. Interestingly, not all granular and semigranular hemocytes stained for crusFpau, revealing that this crustin is produced by specific granule-containing hemocyte subpopulations. Finally, we showed that the granule-stored peptides are not constitutively secreted into the plasma of healthy animals. |
| Databáze: | OpenAIRE |
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