Integrated morphologic and molecular analysis of Trichomonas vaginalis, Mycoplasma hominis, and human papillomavirus using cytologic smear preparations
Autor: | Alexander Muckenhuber, M. Schmidmayr, I. Nikas, R. Götz, M. Bettstetter, D. Angermeier, U. Schenck, Alexander Hapfelmeier, V. Seifert-Klauss, Martin Mollenhauer, Gregor Weirich |
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Rok vydání: | 2018 |
Předmět: |
Adult
DNA Bacterial Sexually transmitted disease Sexual transmission Adolescent Uterine Cervical Neoplasms Mycoplasma hominis medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Trichomonas vaginalis medicine Humans Mycoplasma Infections 030212 general & internal medicine Pap test Papillomaviridae Cervix Microdissection Vaginal Smears Cervical cancer General Veterinary biology medicine.diagnostic_test Coinfection Papillomavirus Infections General Medicine DNA Protozoan biology.organism_classification medicine.disease Virology Infectious Diseases medicine.anatomical_structure 030220 oncology & carcinogenesis Insect Science DNA Viral Female Parasitology Trichomonas Vaginitis Multiplex Polymerase Chain Reaction Papanicolaou Test |
Zdroj: | Parasitology Research. 117:1443-1451 |
ISSN: | 1432-1955 0932-0113 |
DOI: | 10.1007/s00436-018-5829-3 |
Popis: | Pathogenic microbes may colonize the female genital tract via sexual transmission and cause health issues like inflammation or malignancy, summarized as sexually transmitted disease (STD). A major representative of such pathogens is Trichomonas vaginalis (T.v.), whose role in the etiology of cervical cancer remains elusive. Traditional morphologic screening of cervical smears is able to detect T.v., although its identification may be complicated by look-alikes such as degenerated granulocytes and basal cells. In addition, the parasite's endosymbiont Mycoplasma hominis (M.h.) cannot be detected in the Pap test. This investigation was aimed at designing a PCR-based method to detect specific pathogenic germs by using cervical cytology slides to overcome morphologic uncertainty and increase diagnostic accuracy. To test our molecular screening method on T.v., M.h., and HPV in archival smears, we elaborated a multiplex PCR approach based on microdissection. This assay was applied to a minute quantity of starting material which harbored or was suspected to harbor T.v.; the resulting isolated DNA was used for subsequent molecular analyses of T.v., M.h., and HPV. We clarified the diagnosis of genital T.v. infection in 88 and 1.8% of morphologically suspicious and T.v.-negative cases, respectively. We also revealed a tendency of M.h. co-infection in high-risk HPV cases. In conclusion, a microdissection-based approach to detect pathogenic microbes such as T.v., HPV, and M.h. is a molecular tool easy to implement and may help to better understand the interactivity of these germs with respect to pathogenesis. |
Databáze: | OpenAIRE |
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