Analysis of human nasal mucosal cell sheets fabricated using transported tissue and blood specimens
| Autor: | Tsunetaro Morino, Masahiro Takahashi, Masayuki Yamato, Kazuhisa Yamamoto, Hiromi Kojima, Shun Kikuchi, Yuichiro Yaguchi, Ryoto Mitsuyoshi, Yoshiyuki Kasai |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Cell number Autologous blood Mucosal cell Biomedical Engineering Transportation Regenerative medicine Resection Biomaterials 03 medical and health sciences 0302 clinical medicine otorhinolaryngologic diseases Medicine lcsh:QH573-671 lcsh:R5-920 lcsh:Cytology business.industry Cholesteatoma medicine.disease Autologous serum Nasal mucosal cell sheet 030104 developmental biology medicine.anatomical_structure Cell processing center (CPC) Cytokeratin expression Middle ear Original Article lcsh:Medicine (General) business 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Regenerative Therapy, Vol 11, Iss, Pp 88-94 (2019) Regenerative Therapy |
| ISSN: | 2352-3204 |
| DOI: | 10.1016/j.reth.2019.05.001 |
| Popis: | Previously, we succeeded in transplanting autologous nasal mucosal cell sheets in the middle ears of 5 patients, who underwent cholesteatoma resection, which prevents recurrence of cholesteatoma in clinical settings. Current good manufacturing practice (GMP) standards for human cell cultivation requires the establishment of cell processing centers (CPC) which act as germ-free facilities. However, due to practical difficulties involved in establishing and maintaining such facilities at each individual hospital, a functional transport system is felt to be needed for the continuation of effective regenerative therapy. In the current study, nasal mucosal tissue and autologous blood obtained from 3 human volunteers were transported for over 3 h. Disinfected nasal tissues were cultured using keratinocyte culture medium, which included autologous serum prepared from blood. After 24 d, cultured nasal mucosal cells were transported for over 3 h and subsequently assessed for cell number, viability and purity. Moreover, CK4, CK8, and CK18 were analyzed the suitability of these nasal mucosal cell sheets for middle ear regenerative therapy. Overall, we confirmed that nasal mucosal cell sheets can be fabricated using transported nasal mucosal tissue and blood. This study would be contribute to establish a new regenerative therapy for clinical application, accompanied with transportation between companies and hospitals. Highlights • Transportation of nasal mucosal tissue and blood. • Characterizing of nasal mucosal cell sheet by immunohistology. • Transportation of nasal mucosal cell sheet as pre-clinical study. |
| Databáze: | OpenAIRE |
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