A biocompatible open-surface droplet manipulation platform for detection of multi-nucleotide polymorphism
| Autor: | Cheng-Po Huang, Wei-Feng Fang, Jing-Tang Yang, H. Y. E. Chou, M. S. Ke |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Microfluidics Biomedical Engineering Metal Nanoparticles Bioengineering Nanotechnology Biochemistry Contact angle chemistry.chemical_compound Adsorption Deflection (engineering) Materials Testing Humans Dimethylpolysiloxanes Polymorphism Genetic Polydimethylsiloxane Membranes Artificial DNA General Chemistry Microfluidic Analytical Techniques Nylons Membrane chemistry Molecular Probes Reagent Gold Naked eye |
| Zdroj: | Lab Chip. 14:2057-2062 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c4lc00089g |
| Popis: | We present a novel and simple method to manipulate droplets applicable to an open-surface microfluidic platform. The platform comprised a control module for pneumatic droplets and a superhydrophobic polydimethylsiloxane (PDMS) membrane. With pneumatic suction to cause deflection of the flexible PDMS-based superhydrophobic membrane, the sample and reagent droplets on the membrane become transported and mixed. A facile one-step laser micromachining technique serves to fabricate a superhydrophobic surface; a contact angle of 150° and a hysteresis angle of 4° were achieved without chemical modification. Relative to previous open-surface microfluidic systems, this platform is capable of simultaneous and precise delivery of droplets in two-dimensional (2D) manipulation. Droplets were manipulated with suction, which avoided interference from an external driving energy (e.g. heat, light, electricity) to affect the bio-sample inside the droplets. Two common bio-samples, namely protein and DNA, verified the performance of the platform. Based on the experimental results, operations on protein can be implemented without adsorption on the surface of the platform. Another striking result is the visual screening for multi-nucleotide polymorphism with hybridization-mediated growth of gold-nanoparticle (AuNP) probes. The detection results are observable with the naked eye, without the aid of advanced instruments. The entire procedure only takes 5 min from the addition of the sample and reagent to obtaining the results, which is much quicker than the traditional method. The total sample volume consumed in each operation is only 10 μL, which is significantly less than what is required in a large system. According to this approach, the proposed platform is suitable for biological and chemical applications. |
| Databáze: | OpenAIRE |
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