Polyvalent choline phosphate as a universal biomembrane adhesive
| Autor: | Donald E. Brooks, Jayachandran N. Kizhakkedathu, Wei Chen, Xifei Yu, Johan Janzen, Irina Chafeeva, Zonghua Liu, Sonja Horte, Rajesh K. Kainthan |
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| Rok vydání: | 2012 |
| Předmět: |
Erythrocyte Aggregation
Glycerol Models Anatomic endocrine system Erythrocytes Materials science Polymers Phosphorylcholine Lipid Bilayers Static Electricity Biocompatible Materials CHO Cells In Vitro Techniques Membrane Lipids Plasma Cricetulus Adhesives Cricetinae Materials Testing Static electricity Animals Humans Molecule General Materials Science chemistry.chemical_classification Liposome Mechanical Engineering Chinese hamster ovary cell Biological membrane General Chemistry Polymer Condensed Matter Physics Membrane chemistry Biochemistry Mechanics of Materials Microscopy Electron Scanning Adsorption Macromolecule |
| Zdroj: | Nature Materials. 11:468-476 |
| ISSN: | 1476-4660 1476-1122 |
| DOI: | 10.1038/nmat3272 |
| Popis: | Phospholipids in the cell membranes of all eukaryotic cells contain phosphatidyl choline (PC) as the headgroup. Here we show that hyperbranched polyglycerols (HPGs) decorated with the 'PC-inverse' choline phosphate (CP) in a polyvalent fashion can electrostatically bind to a variety of cell membranes and to PC-containing liposomes, the binding strength depending on the number density of CP groups per macromolecule. We also show that HPG-CPs can cause cells to adhere with varying affinity to other cells, and that binding can be reversed by subsequent exposure to low molecular weight HPGs carrying small numbers of PCs. Moreover, PC-rich membranes adsorb and rapidly internalize fluorescent HPG-CP but not HPG-PC molecules, which suggests that HPG-CPs could be used as drug-delivery agents. CP-decorated polymers should find broad use, for instance as tissue sealants and in the self-assembly of lipid nanostructures. |
| Databáze: | OpenAIRE |
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