Carrageenan hydrogel as a scaffold for skin-derived multipotent stromal cells delivery
| Autor: | Andréa Gonçalves Trentin, Adriano Kopke de Aguiar, Maiara Marques da Silva, Rafael Diego Rosa, Felipe Azevedo Gomes, Giordano W. Calloni, Michele Patrícia Rode, Rafael Guzella de Carvalho, Addeli Bez Batti Angulski, Luiz Fernando Cappa Oliveira, Daniel Gonçalves Iucif Vieira, Talita da Silva Jeremias, Lenize Fernandes Maia, Leila Hayashi, Kildare Rocha de Miranda |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Scaffold Stromal cell Biomedical Engineering 02 engineering and technology Carrageenan Mesenchymal Stem Cell Transplantation Biomaterials Extracellular matrix Mice 03 medical and health sciences chemistry.chemical_compound Kappaphycus alvarezii Tissue engineering Animals Humans Cells Cultured Skin Wound Healing Tissue Engineering Tissue Scaffolds integumentary system biology Hydrogels Mesenchymal Stem Cells 021001 nanoscience & nanotechnology biology.organism_classification In vitro Mice Inbred C57BL carbohydrates (lipids) 030104 developmental biology chemistry Self-healing hydrogels Biophysics Female 0210 nano-technology |
| Zdroj: | Journal of Biomaterials Applications. 33:422-434 |
| ISSN: | 1530-8022 0885-3282 |
| Popis: | Carrageenan is a thermoreversible polymer of natural origin widely used in food and pharmaceutical industry that presents a glycosaminoglycan-like structure. Herein, we show that kappa-type carrageenan extracted by a semi-refined process from the red seaweed Kappaphycus alvarezii displayed both chemical and structural properties similar to a commercial carrageenan. Moreover, both extracted carrageenan hydrogel and commercial carrageenan hydrogel can serve as a scaffold for in vitro culture of human skin-derived multipotent stromal cells, demonstrating considerable potential as cell-carrier materials for cell delivery in tissue engineering. Skin-derived multipotent stromal cells cultured inside the carrageenan hydrogels showed a round shape morphology and maintained their growth and viability for at least one week in culture. Next, the effect of the extracted carrageenan hydrogel loaded with human skin-derived multipotent stromal cells was evaluated in a mouse model of full-thickness skin wound. Macroscopic and histological analyses revealed some pointed ameliorated features, such as reduced inflammatory process, faster initial recovery of wounded area, and improved extracellular matrix deposition. These results indicate that extracted carrageenan hydrogel can serve as a scaffold for in vitro growth and maintenance of human SD-MSCs, being also able to act as a delivery system of cells to wounded skin. Thus, evaluation of the properties discussed in this study contribute to a further understanding and specificities of the potential use of carrageenan hydrogel as a delivery system for several applications, further to skin wound healing. |
| Databáze: | OpenAIRE |
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