Anti-inflammatory effects of Mangifera indica L. extract in a model of colitis

Autor: Beatriz G. Pérez-Nievas, Juan C. Leza, Lucía Márquez, Iciar Gárate, José Lm Madrigal, Luis Menchén, Gabino Garrido, Borja García-Bueno
Předmět:
Male
Time Factors
Thiobarbituric acid
Anti-Inflammatory Agents
Administration
Oral
Nitric Oxide Synthase Type II
Pharmacology
Lipid peroxidation
chemistry.chemical_compound
Medicine
Gastrointestinal agent
biology
Dextran Sulfate
Gastroenterology
food and beverages
General Medicine
Colitis
Glutathione
Nitric oxide synthase
Receptors
Tumor Necrosis Factor
Type I
Myeloperoxidase
Original Article
Inflammation Mediators
Colon
digestive system
complex mixtures
Thiobarbituric Acid Reactive Substances
Gastrointestinal Agents
Administration
Rectal
TBARS
Animals
Receptors
Tumor Necrosis Factor
Type II
Rats
Wistar
Mangifera
Interleukin-6
Plant Extracts
Tumor Necrosis Factor-alpha
business.industry
medicine.disease
digestive system diseases
Rats
stomatognathic diseases
Disease Models
Animal
chemistry
Cyclooxygenase 2
Immunology
biology.protein
Lipid Peroxidation
business
Zdroj: King's College London
Popis: To investigate the effect of aqueous extract from Mangifera indica L. (MIE) on dextran sulfate sodium (DSS)-induced colitis in rats.MIE (150 mg/kg) was administered in two different protocols: (1) rectally, over 7 d at the same time as DSS administration; and (2) once daily over 14 d (by oral gavage, 7 d before starting DSS, and rectally for 7 d during DSS administration). General observations of clinical signs were performed. Anti-inflammatory activity of MIE was assessed by myeloperoxidase (MPO) activity. Colonic lipid peroxidation was determined by measuring the levels of thiobarbituric acid reactive substances (TBARS). Reduced glutathione (GSH) levels, expression of inflammatory related mediators [inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, respectively] and cytokines [tumor necrosis factor (TNF)-α and TNF receptors 1 and 2] in colonic tissue were also assessed. Interleukin (IL)-6 and TNF-α serum levels were also measured.The results demonstrated that MIE has anti-inflammatory properties by improvement of clinical signs, reduction of ulceration and reduced MPO activity when administered before DSS. In addition, administration of MIE for 14 d resulted in an increase in GSH and reduction of TBARS levels and iNOS, COX-2, TNF-α and TNF R-2 expression in colonic tissue, and a decrease in IL-6 and TNF-α serum levels.MIE has anti-inflammatory activity in a DSS-induced rat colitis model and preventive administration (prior to DSS) seems to be a more effective protocol.
Databáze: OpenAIRE
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