Thermal annealed silk fibroin membranes for periodontal guided tissue regeneration
Autor: | Ana Oliveira, Cassilda Cunha-Reis, Joaquim M. Oliveira, Silvia Regina Rios Vieira, Ana R. Costa-Pinto, Catarina Geão, Viviana P. Ribeiro, Rui L. Reis |
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Přispěvatelé: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Glycerol
Materials science Hot Temperature Cell Survival Periodontal Ligament Surface Properties 0206 medical engineering Biomedical Engineering Biophysics Fibroin Connective tissue Bioengineering 02 engineering and technology Polyvinyl alcohol Cell Line Biomaterials chemistry.chemical_compound Tensile Strength medicine Cell Adhesion Periodontal fiber Animals Humans Regeneration Cell Proliferation Tissue Scaffolds Regeneration (biology) Membranes Artificial Adhesion Fibroblasts 021001 nanoscience & nanotechnology Bombyx 020601 biomedical engineering Membrane medicine.anatomical_structure chemistry Polyvinyl Alcohol Guided Tissue Regeneration Periodontal Stress Mechanical Swelling medicine.symptom 0210 nano-technology Fibroins Biomedical engineering |
Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
ISSN: | 1573-4838 0957-4530 |
DOI: | 10.1007/s10856-019-6225-y |
Popis: | Guided tissue regeneration (GTR) is a surgical procedure applied in the reconstruction of periodontal defects, where an occlusive membrane is used to prevent the fast-growing connective tissue from migrating into the defect. In this work, silk fibroin (SF) membranes were developed for periodontal guided tissue regeneration. Solutions of SF with glycerol (GLY) or polyvinyl alcohol (PVA) where prepared at several weight ratios up to 30%, followed by solvent casting and thermal annealing at 85 °C for periods of 6 and 12 h to produce high flexible and stable membranes. These were characterized in terms of their morphology, physical integrity, chemical structure, mechanical and thermal properties, swelling capability and in vitro degradation behavior. The developed blended membranes exhibited high ductility, which is particular relevant considering the need for physical handling and adaptability to the defect. Moreover, the membranes were cultured with human periodontal ligament fibroblast cells (hPDLs) up to 7 days. Also, the higher hydrophilicity and consequent in vitro proteolytic degradability of these blends was superior to pure silk fibroin membranes. In particular SF/GLY blends demonstrated to support high cell adhesion and viability with an adequate hPDLs’ morphology, make them excellent candidates for applications in periodontal regeneration. |
Databáze: | OpenAIRE |
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