A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
| Autor: | Sandra Li, Piet Maes, Kalina T. Zlateva, Leen Vijgen, Ben Berkhout, Els Keyaerts, Krzysztof Pyrc, Marc Van Ranst, Lia van der Hoek, Elien Moës |
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| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, Medical Microbiology and Infection Prevention, Faculteit der Geneeskunde |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
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Human coronavirus NL63 medicine.medical_specialty Adolescent viruses Molecular Sequence Data medicine.disease_cause lcsh:Infectious and parasitic diseases Medical microbiology Belgium stomatognathic system Consensus Sequence medicine Humans lcsh:RC109-216 Respiratory system Child Respiratory Tract Infections Phylogeny DNA Primers Coronavirus Base Sequence Respiratory tract infections biology Reverse Transcriptase Polymerase Chain Reaction Incidence Incidence (epidemiology) Infant virus diseases respiratory system biology.organism_classification Virology Infectious Diseases medicine.anatomical_structure Parasitology Child Preschool RNA Viral Female Seasons Coronavirus Infections Sequence Alignment Research Article Respiratory tract |
| Zdroj: | BMC infectious diseases, 5(1). BioMed Central BMC Infectious Diseases CIÊNCIAVITAE BMC Infectious Diseases, 5(6). BioMed Central Scopus-Elsevier BMC Infectious Diseases, Vol 5, Iss 1, p 6 (2005) |
| ISSN: | 1471-2334 |
| Popis: | Background Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium. Methods Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay. Results Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004. Conclusions Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating. |
| Databáze: | OpenAIRE |
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