IL-6 originated from breast cancer tissue-derived mesenchymal stromal cells may contribute to carcinogenesis

Autor: Erdal Karaoz, Özlem Sağlam, Zehra Seda Ünal, Engin Ulukaya, Cansu Subasi
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı., Ulukaya, Engin, K-5792-2018, 2-s2.0-84938199098
Rok vydání: 2014
Předmět:
Cell viability
Carcinogenesis
medicine.medical_treatment
Enzyme linked immunosorbent assay
Mesenchymal stromal cells
Interleukin 6
Cancer cell
Mesenchymal stroma cell
Growth
Real time polymerase chain reaction
medicine.disease_cause
Cancer growth
Spindle cell
chemistry.chemical_compound
Breast cancer
Immunoreactivity
Metasis
Pathology
Tumor Microenvironment
STAT3
Priority journal
Gene expression regulation
Progression
Breast carcinogenesis
Carboxyfluorescein succinimidyl ester
General Medicine
IL6R gene
Stem-cells
Molecular characterization
Gene Expression Regulation
Neoplastic
Cytokine
Oncology
STAT3 protein
human
MCF-7 Cells
Female
Human
Signal Transduction
Glioblastoma
Suicide Genes
Cytosine Deaminase
STAT3 Transcription Factor
Stromal cell
Breast tumor
Breast Neoplasms
IL6 protein
human
Protein targeting
Biology
Biosynthesis
Article
STAT3 gene
STAT3 protein
In-vivo
Genetics
medicine
Humans
Human tissue
Oncogene
Cell Proliferation
IL-6
Neoplastic
Tumor microenvironment
Interleukin-6
Mesenchymal Stem Cells
Coculture
Coculture Techniques
IL6 gene
Metabolism
Human cell
MCF 7 cell line
chemistry
Tumor progression
Cancer research
biology.protein
STAT protein
Protein expression
Gene expression
Zdroj: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(7)
ISSN: 1423-0380
Popis: Tumor microenvironment is an important factor, which sustains and promotes the tumors by inflammatory signals. Interleukin-6 (IL-6) is known as a multifunctional cytokine, which is a major activator of the signaling pathway of Janus kinases (JAKs)/signal transducer and activator of transcription 3 (STAT3). In this study, we aimed to investigate the effect of IL-6 in the tumor microenvironment on carcinogenesis. For this purpose, healthy breast tissue-derived stromal cells (HBT-SCs) and malign breast tissue-derived stromal cells (MBT-SCs) were co-cultured with MCF-7 (human breast adenocarcinoma cell line) cells using semipermeable membranes. The cell proliferation was monitored with water-soluble tetrazolium (WST) and carboxyfluorescein succinimidyl ester (CFSE) assays. Protein levels were measured by enzyme-linked immunosorbent assay (ELISA) and Western blot hybridization, while gene expressions were measured by real-time PCR. The results demonstrated that IL-6 protein levels increased significantly in the supernatants of MBT-SCs when they were co-cultured with MCF-7 cells. In accordance with this, the expression of IL-6 was significantly higher in MBT-SCs. Additionally, the expression of STAT3 in MCF-7 cells increased slightly when they were co-cultured with MBT-SCs. Considering together, there is an important interaction between tumor microenvironment and tumor cells mediated by IL-6 signaling. Thereby, the targeting on IL-6 signaling in the treatment of cancer might effectively prevent the tumor progression.
Databáze: OpenAIRE
Externí odkaz: