Differential Distribution of RBPMS in Pig, Rat, and Human Retina after Damage

Autor: Noelia Ruzafa, Sansar C. Sharma, J. Haritz Urcola, Elena Vecino, Xandra Pereiro
Rok vydání: 2020
Předmět:
0301 basic medicine
inner plexiform layer
RNA binding protein with multiple splicing
genetic structures
Swine
Neurogenesis
Nerve fiber layer
RBPMS
Biology
Retinal ganglion
Catalysis
RNA-Binding Protein With Multiple Splicing
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Ganglion cell layer
lcsh:QH301-705.5
development
ganglion cell layer
Spectroscopy
Cells
Cultured
Retina
CATS
innerplexiform layer
Organic Chemistry
RNA-Binding Proteins
General Medicine
Inner plexiform layer
Axons
Cell Hypoxia
eye diseases
Computer Science Applications
Cell biology
Rats
Protein Transport
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
retinal ganglion cells
sense organs
030217 neurology & neurosurgery
Zdroj: Addi. Archivo Digital para la Docencia y la Investigación
instname
International Journal of Molecular Sciences, Vol 21, Iss 9330, p 9330 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 23
Universidad de Cantabria (UC)
Addi: Archivo Digital para la Docencia y la Investigación
Universidad del País Vasco
ISSN: 2019-1111
Popis: RNA binding protein with multiple splicing (RBPMS) is expressed exclusively in retinal ganglion cells (RGCs) in the retina and can label all RGCs in normal retinas of mice, rats, guinea pigs, rabbits, cats, and monkeys, but its function in these cells is not known. As a result of the limited knowledge regarding RBPMS, we analyzed the expression of RBPMS in the retina of different mammalian species (humans, pigs, and rats), in various stages of development (neonatal and adult) and with different levels of injury (control, hypoxia, and organotypic culture or explants). In control conditions, RBPMS was localized in the RGCs somas in the ganglion cell layer, whereas in hypoxic conditions, it was localized in the RGCs dendrites in the inner plexiform layer. Such differential distributions of RBPMS occurred in all analyzed species, and in adult and neonatal retinas. Furthermore, we demonstrate RBPMS localization in the degenerating RGCs axons in the nerve fiber layer of retinal explants. This is the first evidence regarding the possible transport of RBPMS in response to physiological damage in a mammalian retina. Therefore, RBPMS should be further investigated in relation to its role in axonal and dendritic degeneration. This research was funded by ELKARTEK KK-2019/00086, Research groups of the UPV/EHU (GIU 2018/50)and MINECO-Retos (PID2019-111139RB-I00) to E.V. Programa de perfeccionamiento de personal InvestigadorDoctor, Gobierno Vasco to X.P.
Databáze: OpenAIRE
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