Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis
| Autor: | Atsuko Kamo, Yayoi Kamata, Kyoichi Iizumi, Hiroaki Kawasaki, Ayako Shigenaga, Kenji Takamori, Fumiyuki Yamakura, Mitsutoshi Tominaga, Ayaka Otsu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Medicine (miscellaneous) Immunoglobulin light chain 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot NC/Nga mouse medicine oxidative stress Nutrition and Dietetics biology medicine.diagnostic_test atopic dermatitis Chemistry Nitrotyrosine Atopic dermatitis medicine.disease Molecular biology Blood proteins Nitric oxide synthase 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Biomarker (medicine) biomarker Original Article Antibody 6-nitrotryptophan |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | To reduce the incidence and severity of atopic dermatitis, detection and treatment at an early stage are urgently required, but no effective biomarker has been reported. In this study, we attempted to detect a candidate biomarker of early stage atopic dermatitis by focusing on the levels of nitrated residues in the plasma proteins of atopic dermatitis model mice (NC/Nga mice). We found that the immunoglobulin (Ig) light chain was more highly nitrated in the plasma of the animal model than that of control mice. Western blot analysis showed a statistically significant difference between the 6-nitrotryptophan content of the Ig light chain in the NC/Nga mice before onset of atopic dermatitis symptoms and that of the control mice. LC-ESI-MS/MS analysis demonstrated that these light chains contained nitrotryptophan (Trp56) and nitrotyrosine (Tyr66). Immunofluorescence staining revealed a significant increase in manganese superoxide dismutase and inducible nitric oxide synthase production in the skin lesions of the NC/Nga mice. Furthermore, we found protein-bound 6-nitrotryptophan and 3-nitrotyrosine only in the lesioned skin, where their signals partially overlapped with the IgG signal. Our findings suggest that the 6-nitrotryptophan content of Ig light chains could be a new biomarker for detecting early stage atopic dermatitis. |
| Databáze: | OpenAIRE |
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