Production and assay of ovine T cell growth factor by concanavalin A stimulated peripheral blood leukocytes

Autor:	L. D. Pearson, Keith A. Knisley
Rok vydání:	1987
Předmět:	T-cell growth factor Sheep General Veterinary biology Immunology Proliferation assay Stimulation In Vitro Techniques Autologous serum Lymphocyte Activation Molecular biology Peripheral blood Kinetics Concanavalin A Cell culture biology.protein Leukocytes Animals Interleukin-2 Fetal bovine serum
Zdroj:	Veterinary immunology and immunopathology. 16(1-2)
ISSN:	0165-2427
Popis:	Conditions for the production and assay of ovine T cell growth factor (TCGF) activity were evaluated. Peripheral blood leukocytes were stimulated with concanavalin A (Con A) in the presence of 2% autologous serum or serum-free media. Supernatants were harvested after 30 hr and concentrated for further characterization. A 28 hr proliferation assay with 2.5 × 104 24 hr Con A blasts per well was optimal for detection of TCGF. Peak TCGF activity occurred with a 30–37KD molecular weight fraction. Production and assay of TCGF were performed under autologous conditions to reduce background stimulation which occurred when fetal bovine serum was present. This methodology required no cell lines or inbred animals and should be adaptable to the study of immunostimulatory molecules of other outbred species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a06cc69c72d7e393c9b7424ed606658 https://pubmed.ncbi.nlm.nih.gov/3499702 Zobrazit plný text záznamu