Antisense suppression of S-RNase expression in Nicotiana using RNA polymerase II- and III-transcribed gene constructs
| Autor: | June E. Bourque, Bruce McClure, Jane Murfett |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genetic Vectors
RNA polymerase II Plant Science RNA polymerase III chemistry.chemical_compound Suppression Genetic Transformation Genetic Gene Expression Regulation Plant RNA polymerase Sense (molecular biology) Gene expression Tobacco Genetics RNA Antisense Gene Nicotiana biology Chimera fungi food and beverages RNA Polymerase III General Medicine biology.organism_classification Plants Genetically Modified Molecular biology Antisense RNA Plants Toxic chemistry biology.protein Pollen RNA Polymerase II Agronomy and Crop Science |
| Zdroj: | Plant molecular biology. 29(2) |
| ISSN: | 0167-4412 |
| Popis: | In the Solanaceae, self-incompatibility is controlled by a single, multi-allelic (‘S’) locus. One product of this locus is a ribonuclease, the S-RNase, which is expressed predominantly in mature pistils and has recently been shown to cause allele-specific pollen rejection in transgenic plants. Hybrid Nicotiana plumbaginifolia × N. alata plants were used to test the effects of antisense suppression of the S A2-RNase from N. alata using three different gene constructs: two driven by RNA polymerase II-transcribed promoters, and the third, containing a truncated soybean tRNAmet-i gene, transcribed by RNA polymerase III. All three constructs caused suppression of S-RNase activity in the transgenic plants. Unexpectedly, the CaMV 35S promoter was more effective for antisense suppression than the tissue specific tomato ChiP promoter. Antisense suppression of S-RNase correlated with low sense S A2 transcript levels and high antisense S A2 transcript levels. Untransformed hybrids that contained the N. alata S A2 allele were incompatible with N. alata S A2 pollen, while transgenic plants with suppressed S A2 gene expression accepted the pollen. The utility of this hybrid plant system for studying some aspects of antisense gene suppression is discussed. |
| Databáze: | OpenAIRE |
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