Isolation and characterization of the murine prostate short-chain dehydrogenase/reductase 1 (Psdr1) gene, a new member of the short-chain steroid dehydrogenase/reductase family
| Autor: | Camari Ferguson, Peter S. Nelson, Biaoyang Lin, Colin Pritchard, Stacy Moore |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
7-Dehydrocholesterol reductase DNA Complementary Sequence analysis Molecular Sequence Data Reductase Biology Mice Complementary DNA Genetics Animals Gene family Amino Acid Sequence RNA Messenger Promoter Regions Genetic Gene Phylogeny Short-chain dehydrogenase Base Sequence Sequence Homology Amino Acid Gene Expression Profiling Chromosome Mapping Exons Sequence Analysis DNA General Medicine Blotting Northern Molecular biology Introns Mice Inbred C57BL Genes Female Oxidoreductases Androgen Response Element Sequence Alignment |
| Zdroj: | Gene. 293:149-160 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(02)00718-7 |
| Popis: | We report the isolation and characterization of a complementary DNA (cDNA) encoding a novel member of the short-chain dehydrogenase/reductase (SDR) gene family that we have designated murine prostate short-chain dehydrogenase/reductase 1 (Psdr1). Psdr1 was cloned as a 3.2 kbp transcript from mouse testis cDNA based on the sequence of the recently described androgen-regulated human PSDR1 gene (Cancer Res. 61 (2001) 1611). The putative protein encoded by Psdr1 consists of 316 amino acids with 85% identity to human PSDR1. A search against the BLOCKS database of conserved protein motifs indicates that Psdr1 retains features essential for SDR function. Northern analyses demonstrate that Psdr1 is highly expressed in the murine testis and liver and exhibits several isoforms. Cloning and sequence analysis of the putative Psdr1 promoter region identified motifs with homology to the consensus androgen response element and progesterone response element. The Psdr1 gene was mapped to mouse chromosome 12q31‐34, which has synteny with the human PSDR1 chromosomal location (14q23‐24.3). Together, these data describe a new member of the SDR gene family that may be involved in the tissue-specific metabolism of retinoids or steroid hormones. q 2002 Published by Elsevier Science B.V. |
| Databáze: | OpenAIRE |
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