Stoichiometry of the vinblastine-induced self-association of calf brain tubulin

Autor:	Serge N. Timasheff, George C. Na
Rok vydání:	1980
Předmět:	GTP' Polymers Self association Ligands Vinblastine Models Biological Biochemistry Tubulin medicine Animals Isodesmic reaction biology Chemistry Brain Sedimentation Sedimentation coefficient biology.protein Biophysics Cattle Ultracentrifugation Mathematics Stoichiometry Protein Binding medicine.drug
Zdroj:	Biochemistry. 19:1347-1354
ISSN:	1520-4995 0006-2960
DOI:	10.1021/bi00548a013
Popis:	The self-association of calf brain tubulin in PG buffer (10(-2) M NaPi and 10(-4) M GTP, pH 7.0) induced by the antimitotic drug vinblastine has been investigated by velocity sedimentation. Schlieren sedimentation patterns were examined at low vinblastine concentrations where the boundary resolves into a bimodal one and at high vinblastine concentration where a single forward-skewed peak prevails. Weight-average sedimentation coefficients of tubulin were determined as a function of protein concentration, the results fitting well a self-association model of an idefinite isodesmic mechanism. This was confirmed by computer simulation of the sedimentation boundary profiles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a00fb6cc3e38aad8f2df4bdf5ad6baf https://doi.org/10.1021/bi00548a013 Zobrazit plný text záznamu