Validation and diagnostic efficacy of a lipase assay using the substrate 1,2-o-dilauryl-rac-glycero glutaric acid-(6'methyl resorufin)-ester for the diagnosis of acute pancreatitis in dogs
| Autor: | Sheila McCullough, Anne M. Barger, Roberta Graca, Joanne B. Messick, Walter E. Hoffmann |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Population Sensitivity and Specificity Gastroenterology Substrate Specificity Glutarates Dogs Internal medicine Dig Oxazines Animals Medicine Dog Diseases Lipase Canine pancreatitis education Pancreas education.field_of_study General Veterinary biology Receiver operating characteristic business.industry Reproducibility of Results Reference Standards medicine.disease Hemolysis Endocrinology Pancreatitis biology.protein Acute pancreatitis business |
| Zdroj: | Veterinary Clinical Pathology. 34:39-43 |
| ISSN: | 0275-6382 |
| DOI: | 10.1111/j.1939-165x.2005.tb00007.x |
| Popis: | Background: Increased serum lipase activity has been used historically to support the diagnosis of acute pancreatitis, a common disease in dogs. Most of the lipase assays that are currently in use lack optimum sensitivity and specificity. Objective: The objectives of this study were to 1) validate the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) assay for determination of lipase activity in canine serum and 2) compare results, reference intervals, sensitivity, and specificity of the DGGR assay with a standard 1,2-diglyceride (1,2 DiG) assay for diagnosing acute pancreatitis in dogs. Methods: Precision, linearity, and interference studies were performed for method validation on a Hitachi 911 analyzer. Lipase results from the DGGR and 1,2 DiG assays were compared by linear regression analysis. Sensitivity, specificity, and diagnostic efficacy were determined for both assays on a population of 30 dogs, 15 of which had acute pancreatitis based on history, clinical signs, and ultrasound findings. Results: Within-run and within-day coefficients of variation (CVs) were low ( |
| Databáze: | OpenAIRE |
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