Loss of labeled DNA from rat brain following injections of precursors with high specific radioactivity II. DNA labeled with 5-[131I]iodo-2′-deoxyuridine and 5-[82Br]bromo-2′-deoxyuridine
| Autor: | John C. Cain, Ilmar Merits |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Radioisotopes
Hot Temperature Brain DNA Bromine Chromatography Ion Exchange Nucleic Acid Denaturation Tritium Rat brain Biochemistry Genetics and Molecular Biology (miscellaneous) Molecular biology Deoxyuridine chemistry.chemical_compound Bromodeoxyuridine Biochemistry chemistry Iodine Isotopes Centrifugation Density Gradient Fluorouracil Thymidine Uridine Iodine |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 209:327-338 |
| ISSN: | 0005-2787 |
| Popis: | Intracerebral injections of 10 μC 5-[ 131 I]iodo-2′-deoxyuridine with very high specific radioactivity into adult rats caused the thymidine analog to be rapidly incorporated into brain DNA but the labeled DNA disappeared with an initial apparent half-life time of 6 h. Simultaneous intracerebral administration of 5-fluorouracil increased the incorporation of [ 131 I]iododeoxyuridine but did not influence the decay of labeled DNA. During the loss of the radioactive DNA the amount of unlabeled DNA remained constant. 5-[ 82 Br]Bromo-2′-deoxyuridine of high specific radioactivity was synthesized and used for labeling of rat brain DNA in the presence of 5-fluorouracil. A similar disappearance of labeled DNA was observed with a peak of radioactivity at 3–5 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|