On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum
| Autor: | Paul Mosset, Fadila Benayache, Eric Marchioni, Samir Benayache, Minjie Zhao, Yasmine Chemam |
|---|---|
| Přispěvatelé: | Université frères Mentouri Constantine I (UMC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives, Analyses Physico-Chimiques et Biologiques, Université Mentouri, Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Picein
Phytochemicals Ethyl acetate Pharmaceutical Science antioxidant activity 01 natural sciences Protocatechuic acid Antioxidants Article Analytical Chemistry Helianthemum lcsh:QD241-441 chemistry.chemical_compound Rutin lcsh:Organic chemistry Drug Discovery Vanillic acid Helianthemum ruficomum Cistaceae HPLC-ABTS•+ TEAC ORAC [CHIM]Chemical Sciences Petroleum ether Physical and Theoretical Chemistry Chromatography High Pressure Liquid ComputingMilieux_MISCELLANEOUS Chromatography biology Molecular Structure 010405 organic chemistry Plant Extracts Organic Chemistry Free Radical Scavengers biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Chemistry (miscellaneous) Molecular Medicine Astragalin |
| Zdroj: | Molecules Molecules, 2017, 22 (2), pp.239. ⟨10.3390/molecules22020239⟩ Molecules, MDPI, 2017, 22 (2), pp.239. ⟨10.3390/molecules22020239⟩ Molecules, Vol 22, Iss 2, p 239 (2017) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 2; Pages: 239 |
| ISSN: | 1420-3049 |
| Popis: | Many Helianthemum species (Cistaceae) are recognized for their various medicinal virtues. Helianthemum ruficomum is an endemic species to the septentrional Sahara on which no report is available so far. The purpose of this work was to investigate the chemical composition and the radical scavenging capacity of this species and its isolated components. Collected from Mougheul (south-west of Algeria), the aerial parts were macerated with 80% EtOH/H2O, after evaporation, the remaining extract was diluted with H2O and extracted with petroleum ether, chloroform, ethyl acetate and n-butanol. EtOAc and n-BuOH extracts were evaluated for their free radical scavenging capacity by on-line HPLC-ABTS•+ assay. The obtained data which were confirmed by TEAC and ORAC assays, allowed guiding the fractionation of these extracts by CC, TLC and reverse phase HPLC. Among the components, 14 were isolated and identified by spectroscopic analyses: protocatechuic acid (1), trans-tiliroside (2), cis-tiliroside (3), astragalin (4), picein (7), vanillic acid 4-O-β-d-glucopyranoside (8), lavandoside (9), 4-hydroxybenzoic acid 4-O-β-d-glucopyranoside (10), nicotiflorin (11), rutin (12), vicenin-2 (13), narcissin (14) and stigmasterol (5) and β-sitosterol (6) as a mixture (71% and 29%, respectively). Compounds 5, 7, 8, 9, 10 and 14 were new for the genus Helianthemum. The antioxidant power of all the isolated compounds was also evaluated by HPLC-ABTS•+, TEAC and ORAC assays. The results clearly indicated high antioxidant potential of the extracts and tested compounds of this species especially, compounds 1, 4, 8, 9, 10 and 12. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|