Characterization and biocompatibility of epoxy-crosslinked dermal sheep collagens
| Autor: | van Wachem, P.B., Zeeman, R., Dijkstra, P.J., Feijen, J., Hendriks, Maria A., Hendriks, M., Cahalan, P.T., van Luyn, M.J.A. |
|---|---|
| Přispěvatelé: | Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Faculty of Science and Technology, Biomaterials Science and Technology |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Materials science
Diglycidyl ether Biocompatibility BIOPROSTHESES CARBODIIMIDE Biomedical Engineering Calcification chemistry.chemical_compound Dermis medicine crosslinking CYTOTOXICITY Fibroblast BIOMATERIALS Carbodiimide epoxy compound technology industry and agriculture Biomaterial medicine.disease GLUTARALDEHYDE Epoxy compounds TISSUE REGENERATING CAPACITY medicine.anatomical_structure chemistry Glutaraldehyde Collagen Biomedical engineering Nuclear chemistry Cross-linking |
| Zdroj: | Journal of Biomedical Materials Research, 47(2), 270-277. Wiley Journal of biomedical materials research, 1999(47), 270-277. Wiley |
| ISSN: | 0021-9304 |
| DOI: | 10.1002/(sici)1097-4636(199911)47:2<270::aid-jbm18>3.0.co;2-d |
| Popis: | Dermal sheep collagen (DSC), which was crosslinked with 1,4-butanediol diglycidyl ether (BD) by using four different conditions, was characterized and its biocompatibility was evaluated after subcutaneous implantation in rats. Crosslinking at pH 9.0 (BD90) or with successive epoxy and carbodiimide steps (BD45EN) resulted in a large increase in the shrinkage temperature (T-s) in combination with a clear reduction in amines. Crosslinking at pH 4.5 (BD45) increased the T-s of the material but hardly reduced the number of amines. Acylation (BD45HAc) showed the largest reduction in amines in combination with the lowest T-s. An evaluation of the implants showed that BD45, BD90, and BD45EN were biocompatible. A high influx of polymorphonuclear cells and macrophages was observed for BD45HAc, but this subsided at day 5. At week 6 the BD45 had completely degraded and BD45HAc was remarkably reduced in size, while BD45EN showed a clear size reduction of the outer DSC bundles; BD90 showed none of these features. This agreed with the observed degree of macrophage accumulation and giant cell formation. None of the materials calcified. For the purpose of soft tissue replacement, BD90 was defined as the material of choice because it combined biocompatibility, low cellular ingrowth, low biodegradation, and the absence of calcification with fibroblast ingrowth and new collagen formation. (C) 1999 John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text