Tissue-Engineered Carotid Artery Interposition Grafts Demonstrate High Primary Patency and Promote Vascular Tissue Regeneration in the Ovine Model
| Autor: | Anton G. Kutikhin, Leonid S. Barbarash, A. V. Mironov, Tatiana N. Akentieva, A. R. Shabaev, Maxim Yu. Sinitsky, V. O. Tkachenko, Larisa V. Antonova, Mariam Yu. Khanova, Tatiana V. Glushkova, Vera G. Matveeva, E. A. Velikanova, Nadezhda S. Deeva, V. V. Sevostianova, Evgenia O. Krivkina, Tatiana Yu. Sergeeva, Sergey S. Krutitskiy, M. A. Rezvova, R. A. Mukhamadiyarov |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Vascular smooth muscle Polymers and Plastics primary patency Basic fibroblast growth factor Organic chemistry ovine model Article chemistry.chemical_compound QD241-441 medicine Vascular tissue tissue-engineered vascular graft business.industry carotid artery Regeneration (biology) General Chemistry Heparin Vascular endothelial growth factor chemistry business preclinical development medicine.drug Iloprost |
| Zdroj: | Polymers Volume 13 Issue 16 Polymers, Vol 13, Iss 2637, p 2637 (2021) |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym13162637 |
| Popis: | Tissue-engineered vascular graft for the reconstruction of small arteries is still an unmet clinical need, despite the fact that a number of promising prototypes have entered preclinical development. Here we test Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)Poly(ε-caprolactone) 4-mm-diameter vascular grafts equipped with vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and stromal cell-derived factor 1α (SDF-1α) and surface coated with heparin and iloprost (PHBV/PCL[VEGF-bFGF-SDF]Hep/Ilo, n = 8) in a sheep carotid artery interposition model, using biostable vascular prostheses of expanded poly(tetrafluoroethylene) (ePTFE, n = 5) as a control. Primary patency of PHBV/PCL[VEGF-bFGF-SDF]Hep/Ilo grafts was 62.5% (5/8) at 24 h postimplantation and 50% (4/8) at 18 months postimplantation, while all (5/5) ePTFE conduits were occluded within the 24 h after the surgery. At 18 months postimplantation, PHBV/PCL[VEGF-bFGF-SDF]Hep/Ilo grafts were completely resorbed and replaced by the vascular tissue. Regenerated arteries displayed a hierarchical three-layer structure similar to the native blood vessels, being fully endothelialised, highly vascularised and populated by vascular smooth muscle cells and macrophages. The most (4/5, 80%) of the regenerated arteries were free of calcifications but suffered from the aneurysmatic dilation. Therefore, biodegradable PHBV/PCL[VEGF-bFGF-SDF]Hep/Ilo grafts showed better short- and long-term results than bio-stable ePTFE analogues, although these scaffolds must be reinforced for the efficient prevention of aneurysms. |
| Databáze: | OpenAIRE |
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