The regulatory network for petal anthocyanin pigmentation is shaped by the MYB5a/NEGAN transcription factor in Mimulus
Autor: | Kimmy A Stanton, Arielle M. Cooley, Yao-Wu Yuan, Joshua R. Puzey, Gregory D. Smith, Emily Simmons, Philip A Cheng, Daniel Thomas, Allison Eggert, Kuenzang Om, Xingyu Zheng |
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Rok vydání: | 2021 |
Předmět: |
AcademicSubjects/SCI01140
0106 biological sciences AcademicSubjects/SCI00010 Gene regulatory network Gene Expression Mimulus Flowers color patterning AcademicSubjects/SCI01180 medicine.disease_cause 01 natural sciences anthocyanin differential expression Anthocyanins 03 medical and health sciences monkeyflower feedback loops Gene Expression Regulation Plant RNA interference Gene expression Genetics medicine Gene Regulatory Networks Gene Plant Proteins 030304 developmental biology Investigation 0303 health sciences Mutation biology Pigmentation fungi reaction-diffusion food and beverages Turing network biology.organism_classification Nectar guide AcademicSubjects/SCI00960 Petal evolution of novelty Mendelian trait Transcription Factors 010606 plant biology & botany |
Zdroj: | Genetics |
ISSN: | 1943-2631 |
Popis: | Much of the visual diversity of angiosperms is due to the frequent evolution of novel pigmentation patterns in flowers. The gene network responsible for anthocyanin pigmentation, in particular, has become a model for investigating how genetic changes give rise to phenotypic innovation. In the monkeyflower genus Mimulus, an evolutionarily recent gain of petal lobe anthocyanin pigmentation in M. luteus var. variegatus was previously mapped to genomic region pla2. Here, we use sequence and expression analysis, followed by transgenic manipulation of gene expression, to identify MYB5a—orthologous to the NEGAN transcriptional activator from M. lewisii—as the gene responsible for the transition to anthocyanin-pigmented petals in M. l. variegatus. In other monkeyflower taxa, MYB5a/NEGAN is part of a reaction-diffusion network that produces semi-repeating spotting patterns, such as the array of spots in the nectar guides of both M. lewisii and M. guttatus. Its co-option for the evolution of an apparently non-patterned trait—the solid petal lobe pigmentation of M. l. variegatus—illustrates how reaction-diffusion can contribute to evolutionary novelty in non-obvious ways. Transcriptome sequencing of a MYB5a RNAi line of M. l. variegatus reveals that this genetically simple change, which we hypothesize to be a regulatory mutation in cis to MYB5a, has cascading effects on gene expression, not only on the enzyme-encoding genes traditionally thought of as the targets of MYB5a but also on all of its known partners in the anthocyanin regulatory network. |
Databáze: | OpenAIRE |
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