Bacaba phenolic extract attenuates adipogenesis by down-regulating PPARγ and C/EBPα in 3T3-L1 cells
| Autor: | Monika Schumacher, Lutz Graeve, Judith Lauvai, Fernanda Dias Bartolomeu Abadio Finco |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty PPARγ lcsh:TX341-641 Biology 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Gallic acid Adipogenesis Nutrition and Dietetics Oenocarpus bacaba lcsh:TP368-456 Adiponectin 3T3-L1 cells biology.organism_classification Molecular biology In vitro C/EBPα lcsh:Food processing and manufacture 030104 developmental biology Endocrinology chemistry Apoptosis Cancer cell lcsh:Nutrition. Foods and food supply Intracellular Bacaba (Oenocarpus bacaba Mart.) phenolic extract Food Science |
| Zdroj: | NFS Journal, Vol 9, Iss C, Pp 8-14 (2017) |
| ISSN: | 2352-3646 |
| DOI: | 10.1016/j.nfs.2017.09.001 |
| Popis: | Bacaba ( Oenocarpus bacaba Mart.) is a native Brazilian palm fruit with a high amount of polyphenolics, reported to have an apoptotic effect on cancer cells [1] . Here, we examined the effect of Bacaba phenolic extract (BPE) on adipogenesis using 3T3-L1 preadipocytes. Proliferating and differentiating adipocytes were incubated with BPE at 6, 12, and 24 μg of gallic acid equivalents (GAE)/ml. BPE reduced accumulation of intracellular lipids and protein expression of adipogenic markers including PPARγ, C/EBPα, FABP4, IR-β, and adiponectin in a dose-dependent manner during differentiation of 3T3-L1 cells into adipocytes. Furthermore, lipid accumulation decreased with BPE (24 μg of GAE/ml) during the early stage of mitotic clonal expansion (Days 0–2). In contrast, the inhibition of protein expression of adipogenic markers needed a longer duration (Days 0–4, 0–7, 2–7) of BPE incubation. These results suggest that BPE inhibits adipogenisis in vitro via targeting transcriptional factors during the early and middle stages of differentiation. |
| Databáze: | OpenAIRE |
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