Functional expression of the murine D2, D3and D4dopamine receptors inXenopus laevisoocytes
| Autor: | C. Simone Fishburn, Bo Skaaning Jensen, Sara Fuchs, Berta Levavi-Sivan |
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| Rok vydání: | 1997 |
| Předmět: |
Agonist
Microinjections Sodium-Potassium-Chloride Symporters G protein medicine.drug_class Dopamine Biophysics Xenopus Stimulation Murine D2 dopamine receptor (D2 D3 D4) Transfection Pertussis toxin Biochemistry Receptors Dopamine Xenopus laevis Chlorides GTP-Binding Proteins Structural Biology Genetics medicine Animals Virulence Factors Bordetella Receptor Molecular Biology Bumetanide biology Cell Biology biology.organism_classification Precipitin Tests Molecular biology Gene Expression Regulation Pertussis Toxin Dopamine receptor Oocytes Dopamine Antagonists Xenopus oocyte Carrier Proteins Cotransporter Na+/K+/2Cl− cotransporter Signal Transduction |
| Zdroj: | FEBS Letters. 420:191-195 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(97)01505-6 |
| Popis: | The different murine D2-type dopamine receptors (D2L, D2S, D3L, D3S, and D4) were expressed in Xenopus laevis oocytes. The D2-type receptors were all similarly and efficiently expressed in Xenopus oocytes and were shown to bind the D2 antagonist [125I]sulpride. They were all shown to activate Cl− influx upon agonist stimulation. Using the diagnostic inhibitor bumetanide, we were able to separate the Na+/K+/2Cl− cotransporter component of the Cl− influx from the total unidirectional Cl− influx. The D3L subtype was found to operate exclusively through the bumetanide-insensitive Cl− influx whereas the other D2-type receptors acted on the Na+/K+/2Cl− cotransporter as well. The pertussis toxin sensitivity of the receptor-activated chloride influx via the Na+/K+/2Cl− cotransporter varied between the various D2-type receptors showing that they may couple to different G proteins, and activate different second messenger systems. |
| Databáze: | OpenAIRE |
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