Altered expression of neuroplasticity-related genes in the brain of depressed suicides

Autor: Hooriyah S. Rizavi, Alberto C.C. Frasch, Anabel Alvarez Juliá, G. N. Pandey, Beata Fuchsova
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Adult
Male
medicine.medical_specialty
Proteolipid protein 1
Otras Ciencias Biológicas
Gene Expression
Prefrontal Cortex
Hippocampus
Poison control
Cell Cycle Proteins
Nerve Tissue Proteins
COEXPRESSION ANALYSIS
POSTMORTEM
MRNA EXPRESSION
Article
Ciencias Biológicas
purl.org/becyt/ford/1 [https]
Young Adult
Internal medicine
Neuroplasticity
Gene expression
medicine
CAMK2A
Humans
Myelin Proteolipid Protein
Prefrontal cortex
purl.org/becyt/ford/1.6 [https]
Adaptor Proteins
Signal Transducing
Aged
Depressive Disorder
Major
Membrane Glycoproteins
Neuronal Plasticity
General Neuroscience
Microfilament Proteins
Human brain
Middle Aged
QPCR
Suicide
medicine.anatomical_structure
Endocrinology
Female
GLYCOPROTEIN M6A
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Psychology
Neuroscience
CIENCIAS NATURALES Y EXACTAS
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Background: Expression of the neuronal membrane glycoprotein M6a (GPM6A), the proteolipid protein (PLP/DM20) family member, is downregulated in the hippocampus of chronically stressed animals. Its neuroplastic function involves a role in neurite formation, filopodium outgrowth and synaptogenesis through an unknown mechanism. Disruptions in neuroplasticity mechanisms have been shown to play a significant part in the etiology of depression. Thus, the current investigation examined whether GPM6A expression is also altered in human depressed brain. Methods: Expression levels and coexpression patterns of GPM6A, GPM6B, and PLP1 (two other members of PLP/DM20 family) as well as of the neuroplasticity-related genes identified to associate with GPM6A were determined using quantitative polymerase chain reaction (qPCR) in postmortem samples from the hippocampus (. n=. 18) and the prefrontal cortex (PFC) (. n=. 25) of depressed suicide victims and compared with control subjects (hippocampus n=. 18; PFC n=. 25). Neuroplasticity-related proteins that form complexes with GPM6A were identified by coimmunoprecipitation technique followed by mass spectrometry. Results: Results indicated transcriptional downregulation of GPM6A and GPM6B in the hippocampus of depressed suicides. The expression level of calcium/calmodulin-dependent protein kinase II alpha (CAMK2A) and coronin1A (CORO1A) was also significantly decreased. Subsequent analysis of coexpression patterns demonstrated coordinated gene expression in the hippocampus and in the PFC indicating that the function of these genes might be coregulated in the human brain. However, in the brain of depressed suicides this coordinated response was disrupted. Conclusions: Disruption of coordinated gene expression as well as abnormalities in GPM6A and GPM6B expression and expression of the components of GPM6A complexes were detected in the brain of depressed suicides. Fil: Fuchsova, Beata. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Alvarez Juliá, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Rizavi, H. S.. University of Illinois; Estados Unidos Fil: Frasch, Alberto Carlos C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Pandey, G. N.. University of Illinois; Estados Unidos
Databáze: OpenAIRE
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